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Bioss
CD8 alpha Polyclonal Antibody
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FIGURE: 1 / 4
CD8 alpha Antibody (BS-4791R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG... View More
Product Details
BS-4791R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from rat CD8 alpha.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD8 alpha chain; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha-chain; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta chain; CD8a; CD8A antigen alpha; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen; type I transmembrane glycoprotein
Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37; RHACD8-4
UniProt ID: (Human) P01732, (Human) P10966, (Rat) P07725, (Rat) P05541
Entrez Gene ID: (Chicken) 428800, (Chicken) 403158, (Human) 925, (Human) 926, (Chicken) 396175, (Rat) 24930, (Rat) 24931
T cell mediated immunity
immune response
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
antigen processing and presentation
T cell activation
cytotoxic T cell differentiation
regulation of immune response
positive regulation of calcium-mediated signaling
defense response to virus
adaptive immune response
regulation of defense response to virus by virus
inflammatory response
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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