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Invitrogen
CD8 alpha Polyclonal Antibody
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FIGURE: 1 / 2
CD8 alpha Antibody (PA5-79010) in IHC (P)
Immunohistochemistry (Paraffin) analysis of CD8 in paraffin-embedded section of human tonsil tissue using CD8 alpha Polyclonal Antibody (Product # PA5-79010). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 1 µg/mL dilution overnight at 4... View More
Product Details
PA5-79010
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: JURKAT whole cell, RAJI whole cell, HL-60 whole cell, A549 whole cell, COLO320 whole cell, HELA whole cell. IHC: human tonsil tissue.
Target Information
CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8a molecule; CD8alpha; CD8b; CD8b molecule; CD8beta; cell surface glycoprotein T8 precursor; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; Ly-3 homolog; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein (CD8-beta) precursor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell differentiation antigen Leu-2/T8; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen; unnamed protein product
Gene Aliases: CD8; CD8A; CD8alpha; CD8B; CD8B1; CD8beta; IMD116; LEU2; Ly-3; LY3; LYT3; MAL; p32; P37
UniProt ID: (Human) P01732, (Human) P10966
Entrez Gene ID: (Human) 925, (Human) 926
adaptive immune response
immune system process
immune response
transmembrane receptor protein tyrosine kinase signaling pathway
T cell activation
regulation of immune response
T cell receptor signaling pathway
T cell mediated immunity
cell surface receptor signaling pathway
antigen processing and presentation
cytotoxic T cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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