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Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
- | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
1:200-1:1,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
- | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:200-1:1,000 | View 1 publication 1 publication |
ELISA (ELISA) |
1:10,000 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Mouse |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
2A2 |
Immunogen |
Purified recombinant fragment of CD80 expressed in E. Coli. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
ascites |
Contains |
0.03% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_10981139 |
MA5-15512 targets CD80 in IF and IHC applications and shows reactivity with Human samples.
The MA5-15512 immunogen is purified recombinant fragment of CD80 expressed in E. Coli.
MA5-15512 detects CD80 which has a predicted molecular weight of approximately 55kDa.
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; BB1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1
Gene Aliases: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD80; LAB7
UniProt ID: (Human) P33681
Entrez Gene ID: (Human) 941
Molecular Function:
immunoglobulin receptor superfamily
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