Invitrogen

CD80 Monoclonal Antibody (2A2)

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CD80 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of BCBL-1 cells using CD80/LAB7 monoclonal antibody (Product # MA5-15512) (Green). Blue: DRAQ5 fluorescent DNA dye.

CD80 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human tonsil tissues using CD80/LAB7 monoclonal antibody (Product # MA5-15512).

CD80 Antibody in Western Blot (WB) — Published figure using CD80 monoclonal antibody (Product # MA5-15512) in Western Blot

Product Details

MA5-15512

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:200-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:200-1:1,000	View 1 publication 1 publication
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human
Published species	Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	2A2
Immunogen	Purified recombinant fragment of CD80 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10981139

Product Specific Information

MA5-15512 targets CD80 in IF and IHC applications and shows reactivity with Human samples.

The MA5-15512 immunogen is purified recombinant fragment of CD80 expressed in E. Coli.

MA5-15512 detects CD80 which has a predicted molecular weight of approximately 55kDa.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The protective effects of MSC-EXO against pulmonary hypertension through regulating Wnt5a/BMP signalling pathway.

Journal of cellular and molecular medicine

Zhang Z,Ge L,Zhang S,Wang J,Jiang W,Xin Q,Luan Y

Published figure using CD80 monoclonal antibody (Product # MA5-15512) in Western Blot

Tue Dec 01 00:00:00 EST 2020

Correction of arginine metabolism with sepiapterin-the precursor of nitric oxide synthase cofactor BH₄-induces immunostimulatory-shift of breast cancer.

Biochemical pharmacology

Zheng X,Fernando V,Sharma V,Walia Y,Letson J,Furuta S

MA5-15512 was used in Immunohistochemistry (Paraffin) to reveal that supplementing sepiapterin normalizes arginine metabolism, improves the immunogenicity and inhibits the growth of breast tumor cells.

Mon Jun 01 00:00:00 EDT 2020

Correction of arginine metabolism with sepiapterin-the precursor of nitric oxide synthase cofactor BH₄-induces immunostimulatory-shift of breast cancer.

Biochemical pharmacology

Zheng X,Fernando V,Sharma V,Walia Y,Letson J,Furuta S

Mon Jun 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; BB1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1

Gene Aliases: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD80; LAB7

UniProt ID: (Human) P33681

Entrez Gene ID: (Human) 941

Molecular Function: immunoglobulin receptor superfamily

Function(s)

cell-cell signaling positive regulation of signal transduction regulation of phosphatidylinositol 3-kinase signaling T cell costimulation intracellular signal transduction T cell activation positive regulation of interleukin-2 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of T-helper 1 cell differentiation positive regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of peptidyl-tyrosine phosphorylation cellular response to lipopolysaccharide

Process(es)

virus receptor activity protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity

CD80 Monoclonal Antibody (2A2)

Product Details

MA5-15512

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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