|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG2a, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.125 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.
Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte or splenocyte suspensions. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2.||Mochimaru H,Usui T,Yaguchi T,Nagahama Y,Hasegawa G,Usui Y,Shimmura S,Tsubota K,Amano S,Kawakami Y,Ishida S||Investigative ophthalmology and visual science (49:2172)||2008|
|Not Applicable||Not Cited||Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes.||Yang Z,Day YJ,Toufektsian MC,Xu Y,Ramos SI,Marshall MA,French BA,Linden J||Circulation (114:2056)||2006|
|Not Applicable||Not Cited||Factors associated with severe granulomatous pneumonia in Mycobacterium tuberculosis-infected mice vaccinated therapeutically with hsp65 DNA.||Taylor JL,Ordway DJ,Troudt J,Gonzalez-Juarrero M,Basaraba RJ,Orme IM||Infection and immunity (73:5189)||2005|
|Not Applicable||Not Cited||Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells.||Grabbe S,Varga G,Beissert S,Steinert M,Pendl G,Seeliger S,Bloch W,Peters T,Schwarz T,Sunderkötter C,Scharffetter-Kochanek K||The Journal of clinical investigation (109:183)||2002|
|Not Applicable||Not Cited||Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.||Ledbetter JA,Herzenberg LA||Immunological reviews (47:63)||1980|
|Not Applicable||Not Cited||T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.||Ledbetter JA,Rouse RV,Micklem HS,Herzenberg LA||The Journal of experimental medicine (152:280)||1980|