Invitrogen

CD9 Monoclonal Antibody (Ts9)

Product Image Gallery

CD9 Antibody — Fig. 1 Characterization of exosomes from HIV-infected T cells. a Transmission electron microscope (TEM) images of exosomes isolated from T-cell culture supernatants. The representative J1.1 cell exosome image is shown. Scale bar, 100 nm. b Immunoblot of CD63, CD9, and CD81 on proteins extracted from T-cell line exosomes (whole scans of blots in Supplementary Figure 1). c CD63 and CD9 immunoblot (left) and AChE assays (right) of J1.1 exosomes isolated from 2, 10, and 20 ml of culture supernatants. Error bars, +- s.d. Data shown one experiment from three biological repeats. Numbers of exosomes (#Exo) were calculated by AChE activity using a standard provided by SBI

Product Details

Tested Applications

Dilution

Western Blot (WB)

1 µg/mL

Published Applications

Western Blot (WB)

See 4 publications below

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Ts9

Immunogen

HeLa (human ovarian cancer cell line)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_2532982

Product Specific Information

The Exosome - anti-CD9 antibody recognizes the CD9/p24 antigen (~24 kDa), a member of the tetraspanin family. The antibody has been verified for western blotting of cellular and exosomal CD9 antigen.

Target Information

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. CD9 belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression plays a critical role in the suppression of cancer cell motility and metastasis. CD9 is also considered as metastasis suppressor in solid tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 5H9; 5H9 antigen; antigen CD9; BA-2/p24 antigen; BA2; BTCC-1; CD9; CD9 antigen; CD9 antigen (p24); Cell growth-inhibiting gene 2 protein; DRAP-27; GIG2; Leukocyte antigen MIC3; MIC3; motility related protein-1; Motility-related protein; MRP-1; P24; Tetraspanin-29; TSPAN-29

Gene Aliases: BTCC-1; CD9; DRAP-27; GIG2; MIC3; MRP-1; TSPAN-29; TSPAN29

UniProt ID: (Human) P21926

Entrez Gene ID: (Human) 928

Molecular Function: cell adhesion molecule membrane-bound signaling molecule receptor signaling molecule