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      CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PE, eBioscience™

      See 46 other FAS antibodies ›
      CD95 (APO-1/Fas) Monoclonal Antibody, PE conjugate for Flow

      Catalog#: 12-0951-81 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      12-0951-81 50 µg
      99.00
      12-0951-83 200 µg
      279.00
      Please enter a quantity in the range of 1 to 199.
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      Product Details

      Bioinformatics

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      Details
      Tested species reactivity Mouse
      Host / Isotype Mouse / IgG1, kappa
      Class Monoclonal
      Type Antibody
      Clone 15A7
      Conjugate PE
      Form Liquid
      Concentration 0.2 mg/mL
      Purification Affinity chromatography
      Storage buffer PBS, pH 7.2, with 0.1% gelatin
      Contains 0.09% sodium azide
      Storage Conditions 4° C, store in dark, DO NOT FREEZE!
      Tested Applications Dilution *
      Flow Cytometry (Flow) 1 µg/test

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Product Specific Information

      Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

      Applications Reported: The 15A7 antibody has been reported for use in flow cytometric analysis.

      Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of BALB/c splenocytes and thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

      Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

      Background/Target Information

      CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      Bioinformatics

      Protein Aliases:

      ALPS1A; APO-1; Apo-1 antigen; Apo-1 Fas; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FAS1; FASLG receptor; FASTM; lymphoproliferation; TNFRSF6; Tumor necrosis factor receptor superfamily member 6

      Gene Aliases:

      AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

      UniProt ID:

      (Mouse) P25446

      Entrez Gene ID:

      (Mouse) 14102

      Molecular Function:

      cytokine receptor receptor tumor necrosis factor receptor

      Function(s) Process(es)

      Documents for

      Material safety data sheets (MSDS)

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