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      View (45) other FAS (CD95) antibodies

      Invitrogen

      CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PE, eBioscience™

      Published Figures 2 Published Figures
      References Icon 6 References
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      Catalog # 12-0951-81

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      Product Details

      TESTED APPLICATIONS DILUTION
      Flow Cytometry (Flow) 1 µg/test
      PUBLISHED APPLICATIONS
      Flow Cytometry (Flow) See 6 publications below
      Species reactivity Mouse
      Published species Mouse
      Host / Isotype Mouse / IgG1, kappa
      Recommended Isotype Control Mouse IgG1 kappa Isotype Control, PE, eBioscience™
      Class Monoclonal
      Type Antibody
      Clone 15A7
      Conjugate PE
      Excitation/Emission Profile View spectra
      Form Liquid
      Concentration 0.2 mg/mL
      Purification Affinity chromatography
      Storage buffer PBS, pH 7.2, with 0.1% gelatin
      Contains 0.09% sodium azide
      Storage conditions 4° C, store in dark, DO NOT FREEZE!
      RRID AB_465788

      Product Specific Information

      Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

      Applications Reported: The 15A7 antibody has been reported for use in flow cytometric analysis.

      Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of BALB/c splenocytes and thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

      Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

      Filtration: 0.2 µm post-manufacturing filtered.

      Background/Target Information

      CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References (6)

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      Bioinformatics

      Protein Aliases: ALPS1A; APO-1; Apo-1 antigen; Apo-1 Fas; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FAS1; FASLG receptor; FASTM; lymphoproliferation; TNFRSF6; Tumor necrosis factor receptor superfamily member 6

      Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

      UniProt ID: (Mouse) P25446

      Entrez Gene ID: (Mouse) 14102

      Molecular Function: cytokine receptor receptor tumor necrosis factor receptor

      Function(s) Blue Arrow Icon
      immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
      Process(es) Blue Arrow Icon
      protease binding
       transmembrane signaling receptor activity
       tumor necrosis factor-activated receptor activity
       protein binding
       kinase binding
       protein complex binding
       identical protein binding

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