Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PE, eBioscience™

Product Image Gallery

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow) — BALB/c mouse thymocytes were stained with 1.0 µg of Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (blue histogram) or 1.0 µg of CD95 (APO-1/Fas) Monoclonal Antibody, PE (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

Product Details

12-0951-81

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	1 µg/test	View 29 publications 29 publications
Functional Assay (FN)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	15A7
Conjugate	PE Alexa Fluor 488 APC Biotin PerCP-eFluor 710 Super Bright 436 Unconjugated
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_465788

Product Specific Information

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported: The 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of BALB/c splenocytes and thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunity.

NPJ vaccines

Hendin HE,Lavoie PO,Gravett JM,Pillet S,Saxena P,Landry N,D'Aoust MA,Ward BJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Mon Apr 11 00:00:00 EDT 2022

Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.

Journal of translational medicine

Park JS,Lee D,Yang S,Jeong HY,Na HS,Cho KH,Choi J,Koo H,Cho ML,Park SH

12-0951-81 was used in Flow Cytometry to study the efficacy of methotrexate-loaded nanoparticles against experimental model of rheumatoid arthritis.

Fri Feb 11 00:00:00 EST 2022

Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway.

Scientific reports

Kim HJ,Kim YJ,Park W

12-0951-81 was used in Flow Cytometry to show that berberine has anti-inflammatory properties related to its inhibition of NO, PGE2, Fas, GM-CSF, LIF, LIX, RANTES, and MIP-2 in dsRNA-induced macrophages via the endoplasmic reticulum stress-related calcium-.

Fri May 28 00:00:00 EDT 2021

Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway.

Scientific reports

Kim HJ,Kim YJ,Park W

Fri May 28 00:00:00 EDT 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Ascorbic Acid Promotes Plasma Cell Differentiation through Enhancing TET2/3-Mediated DNA Demethylation.

Cell reports

Qi T,Sun M,Zhang C,Chen P,Xiao C,Chang X

12-0951 was used in Flow cytometry/Cell sorting to show that ascorbic acid (vitamin C), an essential nutrient, is able to promote plasma cell differentiation and humoral immune response by enhancing TET2/3-mediated DNA demethylation.

Tue Dec 01 00:00:00 EST 2020

A Recombinant Rabies Virus Expressing Fms-like Tyrosine Kinase 3 Ligand (Flt3L) Induces Enhanced Immunogenicity in Mice.

Virologica Sinica

Zhang Y,Yang J,Li M,Cui M,Fu ZF,Zhao L,Zhou M

12-0951 was used in Flow cytometry/Cell sorting to extend the potential of Flt3L as a good adjuvant to develop novel rabies vaccine by enhancing the VNA production through activating the DC-TFH-GC B axis in immunized mice.

Sun Dec 01 00:00:00 EST 2019

The chemokine receptor CCR7 is a promising target for rheumatoid arthritis therapy.

Cellular & molecular immunology

Moschovakis GL,Bubke A,Friedrichsen M,Ristenpart J,Back JW,Falk CS,Kremmer E,Förster R

12-0951-81 was used in Flow Cytometry to demonstrate that CCR7 signaling is essential for the induction of CIA and identify CCR7 as a potential therapeutic target in RA.

Tue Oct 01 00:00:00 EDT 2019

Cyclooxygenase-1 Regulates the Development of Follicular Th Cells via Prostaglandin E₂.

Journal of immunology (Baltimore, Md. : 1950)

Liu T,Yang Q,Cao YJ,Yuan WM,Lei AH,Zhou P,Zhou W,Liu YD,Shi MH,Yang Q,Tang JY,Wang HK,Zhang H,Yu Y,Zhou J

12-0951-81 was used in Flow Cytometry to indicate that COX-1 plays an important role in the development of TFH cells.

Thu Aug 15 00:00:00 EDT 2019

T-Bet⁺ IgM Memory Cells Generate Multi-lineage Effector B Cells.

Cell reports

Kenderes KJ,Levack RC,Papillion AM,Cabrera-Martinez B,Dishaw LM,Winslow GM

12-0951 was used in Flow cytometry/Cell sorting to propose that T-bet+ IgM memory cells can differentiate into any effector B cell lineage to provide long-term multifunctional immunity.

Tue Jul 24 00:00:00 EDT 2018

Human herpesvirus-encoded kinase induces B cell lymphomas in vivo.

The Journal of clinical investigation

Anders PM,Montgomery ND,Montgomery SA,Bhatt AP,Dittmer DP,Damania B

12-0951 was used in Flow cytometry/Cell sorting to analyse the function of viral protein kinase in vivo.

Fri Jun 01 00:00:00 EDT 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment.

Scientific reports

Yeku OO,Purdon TJ,Koneru M,Spriggs D,Brentjens RJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

Clonal Evolution of Autoreactive Germinal Centers.

Cell

Degn SE,van der Poel CE,Firl DJ,Ayoglu B,Al Qureshah FA,Bajic G,Mesin L,Reynaud CA,Weill JC,Utz PJ,Victora GD,Carroll MC

12-0951 was used in Flow cytometry/Cell sorting to study the maturation of the self-reactive B cell response, contextualising the epitope spreading observed in autoimmune disease.

Thu Aug 24 00:00:00 EDT 2017

Loss of IP₃ Receptor-Mediated Ca²⁺ Release in Mouse B Cells Results in Abnormal B Cell Development and Function.

Journal of immunology (Baltimore, Md. : 1950)

Tang H,Wang H,Lin Q,Fan F,Zhang F,Peng X,Fang X,Liu J,Ouyang K

12-0951 was used in Flow cytometry/Cell sorting to determine the function of 1,4,5-trisphosphate receptor-mediated Ca2+ release in B cells.

Sat Jul 15 00:00:00 EDT 2017

Regulation of endothelial Fas expression as a mechanism of promotion of vascular integrity by mural cells in tumors.

Cancer science

Kamei R,Tanaka HY,Kawano T,Morii C,Tanaka S,Nishihara H,Iwata C,Kano MR

12-0951 was used in Flow cytometry/Cell sorting to explore the relationship between endothelial Fas expression, mural cells and vascular integrity in tumour angiogenesis.

Mon May 01 00:00:00 EDT 2017

The IgM receptor FcμR limits tonic BCR signaling by regulating expression of the IgM BCR.

Nature immunology

Nguyen TT,Kläsener K,Zürn C,Castillo PA,Brust-Mascher I,Imai DM,Bevins CL,Reardon C,Reth M,Baumgarth N

12-0951 was used in Flow cytometry/Cell sorting to study the expression and role of FcμR in the trans-Golgi network of developing B cells.

Wed Mar 01 00:00:00 EST 2017

A Novel Rabies Vaccine Expressing CXCL13 Enhances Humoral Immunity by Recruiting both T Follicular Helper and Germinal Center B Cells.

Journal of virology

Wang Z,Li M,Zhou M,Zhang Y,Yang J,Cao Y,Wang K,Cui M,Chen H,Fu ZF,Zhao L

12-0951-81 was used in Functional assay to provide a better understanding of the role of CXCL13 in RABV-induced immune responses which will help in designing more efficacious rabies vaccines.

Wed Feb 01 00:00:00 EST 2017

YY1 plays an essential role at all stages of B-cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Kleiman E,Jia H,Loguercio S,Su AI,Feeney AJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Tue Jul 05 00:00:00 EDT 2016

Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells.

PloS one

Kim YU,Lim H,Jung HE,Wetsel RA,Chung Y

12-0951 was used in Flow cytometry/Cell sorting to demonstrate that Tfh cells rather than Th17 cells contribute to the autoimmune germinal center reactions in BXD2 mice.

Fri Jan 29 00:00:00 EST 2016

Cancer immunotherapy employing an innovative strategy to enhance CD4+ T cell help in the tumor microenvironment.

PloS one

Song L,Yang MC,Knoff J,Wu TC,Hung CF

12-0951 was used in Flow cytometry/Cell sorting to investigate the effect of intratumoral injection of PADRE and the adjuvant CpG into HPV16 E7-expressing TC-1 tumours following cisplatin chemotherapy, showing potent antitumor effects and antigen-specific cell-mediated immune response.

Tue Dec 15 00:00:00 EST 2015

The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development.

Nature medicine

Ortega-Molina A,Boss IW,Canela A,Pan H,Jiang Y,Zhao C,Jiang M,Hu D,Agirre X,Niesvizky I,Lee JE,Chen HT,Ennishi D,Scott DW,Mottok A,Hother C,Liu S,Cao XJ,Tam W,Shaknovich R,Garcia BA,Gascoyne RD,Ge K,Shilatifard A,Elemento O,Nussenzweig A,Melnick AM,Wendel HG

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age.

The Journal of biological chemistry

Zhao H,Bauzon F,Bi E,Yu JJ,Fu H,Lu Z,Cui J,Jeon H,Zang X,Ye BH,Zhu L

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Fri Feb 27 00:00:00 EST 2015

Coinfection with Streptococcus pneumoniae modulates the B cell response to influenza virus.

Journal of virology

Wolf AI,Strauman MC,Mozdzanowska K,Whittle JR,Williams KL,Sharpe AH,Weiser JN,Caton AJ,Hensley SE,Erikson J

12-0951 was used in Flow cytometry/Cell sorting to demonstrate how co-infection of S. pneumoniae and influenza virus may affect the generation of protective antiviral antibodies.

Wed Oct 01 00:00:00 EDT 2014

Interleukin-21 promotes germinal center reaction by skewing the follicular regulatory T cell to follicular helper T cell balance in autoimmune BXD2 mice.

Arthritis & rheumatology (Hoboken, N.J.)

Ding Y,Li J,Yang P,Luo B,Wu Q,Zajac AJ,Wildner O,Hsu HC,Mountz JD

12-0951 was used in Flow cytometry/Cell sorting to indicate that high levels of IL-21 selectively enhance Tfh cell differentiation but inhibit Tfr cell commitment and the suppressive function of Tfr cells on Tfh cells and B cells.

Mon Sep 01 00:00:00 EDT 2014

Up-regulated expression of miR-23a/b targeted the pro-apoptotic Fas in radiation-induced thymic lymphoma.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Li B,Sun M,Gao F,Liu W,Yang Y,Liu H,Cheng Y,Liu C,Cai J

12-0951 was used in Flow cytometry/Cell sorting to demonstrate that the expression of miR-23a/b is up-regulated in radiation-induced thymic lymphoma.

Fri Aug 29 00:00:00 EDT 2014

IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

Journal of immunology (Baltimore, Md. : 1950)

Ding Y,Li J,Wu Q,Yang P,Luo B,Xie S,Druey KM,Zajac AJ,Hsu HC,Mountz JD

12-0951 was used in Flow cytometry/Cell sorting to investigate the role of germinal centres in autoimmunity, showing that IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

Thu Aug 15 00:00:00 EDT 2013

B cell receptor revision diminishes the autoreactive B cell response after antigen activation in mice.

The Journal of clinical investigation

Wang YH,Diamond B

12-0951 was used in Flow cytometry/Cell sorting to suggest that tolerance occurs post-activation in B cells and that B cell receptor revision regulates autoreactivity generated during an immune response.

Fri Aug 01 00:00:00 EDT 2008

Characterization of fibroblasts responsible for cartilage destruction in arthritis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U

12-0951 was used in Flow cytometry/Cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis.

Tue Apr 01 00:00:00 EDT 2008

Up-regulation of Fas (CD95) expression in tumour cells in vivo.

Immunology

Peshes-Yaloz N,Rosen D,Sondel PM,Krammer PH,Berke G

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 12-0951-81) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Apo-1 antigen; Apo-1 Fas; Apo1 antigen; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; Tumor necrosis factor receptor superfamily member 6

Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

UniProt ID: (Mouse) P25446

Entrez Gene ID: (Mouse) 14102

Molecular Function: transmembrane signal receptor

Function(s)

immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

Process(es)

protease binding transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), PE, eBioscience™

Product Details

12-0951-81

Flow Cytometry (Flow)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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