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Invitrogen
CDK4 Polyclonal Antibody
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FIGURE: 1 / 8
CDK4 Antibody (PA5-79025) in IHC (P)
Immunohistochemistry (Paraffin) analysis of CDK4 in paraffin-embedded section of human colon cancer tissue using CDK4 Polyclonal Antibody (Product # PA5-79025). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 5 µg/mL dilution overnight at... View More
Product Details
PA5-79025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Cdk4 recombinant protein (Position: G201-E303).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human K562 whole cell, rat PC-12 whole cell, mosue NIH/3T3 whole cell, mosue RAW2647 whole cell. IHC: mouse intestine tissue, rat intestine tissue, human lung cancer tissue.
Target Information
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:
CDK4; Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; orf; p34
Gene Aliases: CDK4; CMM3; Crk3; PSK-J3
UniProt ID: (Human) P11802, (Rat) P35426, (Mouse) P30285
Entrez Gene ID: (Human) 1019, (Rat) 94201, (Mouse) 12567
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
cyclin-dependent protein serine/threonine kinase regulator activity
transferase activity
cyclin binding
protein serine kinase activity
protein complex binding
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
signal transduction
positive regulation of cell proliferation
response to xenobiotic stimulus
regulation of G2/M transition of mitotic cell cycle
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of fibroblast proliferation
cell division
regulation of cell cycle
regulation of transcription initiation from RNA polymerase II promoter
regulation of type B pancreatic cell proliferation
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to phorbol 13-acetate 12-myristate
cellular response to ionomycin
lens development in camera-type eye
protein phosphorylation
circadian rhythm
response to toxic substance
response to organic substance
response to lead ion
organ regeneration
response to testosterone
regulation of cell proliferation
response to drug
positive regulation of apoptotic process
positive regulation of translation
positive regulation of cell size
response to hyperoxia
negative regulation of cell cycle arrest
cell cycle
phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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