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Invitrogen
CDK4 Polyclonal Antibody
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Product Details
PA5-85345
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human CDK4. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.82 mg/mL
Amount
82 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (95%), Rat (95%), Pig (98%), Sheep (96%), Rhesus Monkey (100%), Bovine (96%).
Positive Control: 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK4; Cell division protein kinase 4; Cyclin-dependent kinase 4; MGC14458; orf; PSK-J3; unnamed protein product
Gene Aliases: CDK4; CMM3; PSK-J3
UniProt ID: (Human) P11802
Entrez Gene ID: (Human) 1019
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
cyclin-dependent protein serine/threonine kinase regulator activity
transferase activity
cyclin binding
protein serine kinase activity
G1/S transition of mitotic cell cycle
signal transduction
positive regulation of cell proliferation
response to xenobiotic stimulus
regulation of G2/M transition of mitotic cell cycle
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of fibroblast proliferation
cell division
regulation of cell cycle
regulation of transcription initiation from RNA polymerase II promoter
regulation of type B pancreatic cell proliferation
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to phorbol 13-acetate 12-myristate
cellular response to ionomycin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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