Invitrogen

CDX2 Polyclonal Antibody

Product Image Gallery

CDX2 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of HT-29 cells using CDX2 Polyclonal antibody (Product # PA5-95733) at dilution of 1:100. Blue: DAPI for nuclear staining.

CDX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human esophageal cancer using CDX2 Polyclonal antibody (Product # PA5-95733) at a dilution of 1:100 (40x lens).

CDX2 Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using CDX2 Polyclonal antibody (Product # PA5-95733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:50 - 1:200

Immunofluorescence (IF)

1:50 - 1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human CDX2.

Conjugate

Unconjugated

Form

Liquid

Concentration

3.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MYVSYLLDKD VSMYPSSVRH SGGLNLAPQN FVSPPQYPDY GGYHVAAAAA AAANLDSAQS PGPSWPAAYG APLREDWNGY APGGAAAAAN AVAHGLNGGS PAAAMGYSSP ADYHPHHHPH HHPHHPAAAP SCASGLLQTL NPGPPGPAAT AAAEQLSPGG QRRNLCEWMR KPAQQSLGSQ; Positive Samples: SW620, MCF7; Cellular Location: Nucleus

Target Information

The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: caudal type homeo box 2; caudal type homeo box transcription factor 2; caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX-3; Homeobox protein CDX-2; homeobox protein miniCDX2

Gene Aliases: Cdx-2; CDX-3; CDX2; CDX2/AS; CDX3

UniProt ID: (Human) Q99626, (Mouse) P43241

Entrez Gene ID: (Human) 1045, (Mouse) 12591, (Rat) 66019

Molecular Function: DNA binding protein helix-turn-helix transcription factor homeobox transcription factor nucleic acid binding transcription factor