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CERT Polyclonal Antibody
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FIGURE: 1 / 14
CERT Antibody (PA5-115035) in ICC/IF
Immunocytochemistry analysis of CERT in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115035) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115035
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human COL4A3BP(Accession Q9Y5P4), corresponding to amino acid residues K284-D334.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total COL4A3BP.
Target Information
CERT (GPBP) is a GC-rich promotor-specific transactivating transcription factor. This protein is suggested to play a role in the development of atherosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha 3 type IV collagen binding protein; ceramide transport protein; collagen type IV alpha 3 binding protein; collagen type IV alpha-3-binding protein; collagen, type IV, alpha 3 (Goodpasture antigen) binding protein; goodpasture antigen-binding protein; GPBP; kinase; lipid-transfer protein CERTL; procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein; StAR-related lipid transfer (START) domain containing 11; stAR-related lipid transfer protein 11; stARD11; START domain-containing protein 11; unnamed protein product
Gene Aliases: 2810404O15Rik; 9230101K08Rik; CERT; CERTL; COL4A3BP; GPBP; MRD34; NEDHSF; STARD11
UniProt ID: (Human) Q9Y5P4, (Mouse) Q9EQG9
Entrez Gene ID: (Human) 10087, (Mouse) 68018, (Rat) 365652
ceramide metabolic process
sphingomyelin biosynthetic process
immune response
sphingolipid biosynthetic process
ER to Golgi ceramide transport
intermembrane lipid transfer
intermembrane sphingolipid transfer
cell morphogenesis
in utero embryonic development
heart morphogenesis
muscle contraction
mitochondrion organization
endoplasmic reticulum organization
signal transduction
cell proliferation
response to endoplasmic reticulum stress
lipid homeostasis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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