Proteintech

CHOP Polyclonal Antibody

View all (37) CHOP antibodies

Questions & Answers

Questions & Answers

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CHOP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer using 15204-1-AP (CHOP antibody) at dilution of 1:50 (under 10x lens).

CHOP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer using 15204-1-AP (CHOP antibody) at dilution of 1:50 (under 40x lens).

CHOP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 15204-1-AP (CHOP antibody) at dilution of 1:50 (under 40x lens).

CHOP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 15204-1-AP (CHOP antibody) at dilution of 1:50 (under 10x lens).

CHOP Antibody in Western Blot (WB) — Tunicamycin treated Hela cells were subjected to SDS PAGE followed by western blot with 15204-1-AP ( CHOP antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

CHOP Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CHOP Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 15204-1-AP (CHOP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CHOP Antibody in Western Blot (WB) — WB result of CHOP antibody (15204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHOP transfected HeLa cells.

CHOP Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug CHOP antibody (15204-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 69 publications 69 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHOP Fusion Protein Ag7354

Conjugate

Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C/EBP homologous protein; C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; Growth arrest and DNA damage-inducible protein GADD153; Growth arrest and DNA-damage-inducible protein GADD153; MGC4154

Gene Aliases: CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153

UniProt ID: (Human) P35638, (Mouse) P35639

Entrez Gene ID: (Human) 1649, (Mouse) 13198, (Rat) 29467

negative regulation of transcription from RNA polymerase II promoter blood vessel maturation transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process ER overload response response to unfolded protein cell cycle cell cycle arrest Wnt signaling pathway endoplasmic reticulum unfolded protein response positive regulation of interleukin-8 production negative regulation of CREB transcription factor activity response to endoplasmic reticulum stress response to starvation mRNA transcription from RNA polymerase II promoter positive regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process regulation of transcription involved in anterior/posterior axis specification cell redox homeostasis negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter release of sequestered calcium ion into cytosol negative regulation of protein kinase B signaling intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of canonical Wnt signaling pathway positive regulation of neuron death negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress negative regulation of determination of dorsal identity response to amphetamine cellular response to DNA damage stimulus aging response to nutrient PERK-mediated unfolded protein response ATF6-mediated unfolded protein response response to drug response to hydrogen peroxide regulation of DNA-templated transcription in response to stress embryonic organ development positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway response to oxidative stress

Process(es)

transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding cAMP response element binding protein binding protein homodimerization activity leucine zipper domain binding transcription regulatory region DNA binding protein heterodimerization activity sequence-specific DNA binding RNA polymerase II transcription factor binding