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Invitrogen
CHRNA5 Polyclonal Antibody
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FIGURE: 1 / 9
CHRNA5 Antibody (PA5-79046) in IHC (P)
Immunohistochemistry analysis of CHRNA5 on paraffin-embedded human prostatic cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with CHRNA5 polyclonal antibody (Product# PA5-79046) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37... View More
Product Details
PA5-79046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CHRNA5 (44-76aa AKHEDSLLKDLFQDYERWVRPVEHLNDKIKIKF).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat skeletal muscle tissue, HEPG2 whole cell. IHC: mouse intestine tissue, mouse cardiac muscle tissue, rat intestine tissue, rat cardiac muscle tissue, human prostatic cancer tissue. Flow: U251 cell, A549 cell, MCF-7 cell.
Target Information
Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetylcholine receptor; acetylcholine receptor alpha 5; acetylcholine receptor, nicotinic, alpha 5 (neuronal); cholinergic receptor; Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5; cholinergic receptor, nicotinic alpha 5; cholinergic receptor, nicotinic, alpha 5 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 5; nAChR alpha 5; Neuronal acetylcholine receptor subunit alpha-5; neuronal nicotinic acetylcholine receptor, alpha5 subunit; nicotinic acetylcholine receptor alpha5 subunit
Gene Aliases: Acra-5; Acra5; CHRNA5; LNCR2; NACHRA5
UniProt ID: (Human) P30532, (Mouse) Q2MKA5
Entrez Gene ID: (Human) 1138, (Mouse) 110835, (Rat) 25102
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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