|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||Protein corresponding to residues 264-361 from the C terminus of human Chx10, conjugated to GST.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||0.5-1µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
By Western blot, this antibody detects a ~46 kDa band. A suggested positive control for this product is rat or mouse retinal tissue lysate.
Chx10 is a 46kDa homeodomain protein of the paried-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalmus, and spinal cord.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.