Invitrogen

CK1 delta Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (12) CK1 delta antibodies

Datasheet

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Datasheet

Questions & Answers

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CK1 delta Antibody in Western Blot (WB) — Knockdown of CK1 delta was achieved by transfecting HeLa with CK1 delta specific siRNAs (Silencer® select Product # s3627 and s3628). Western blot analysis (Fig. a) was performed using Whole cell extracts from the CK1 delta knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CK1 delta Polyclonal Antibody (Product # PA5-18509, 0.75 µg/ml) and Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CK1 delta.

CK1 delta Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of CK1 delta in U251 cells using a CK1 delta polyclonal antibody (Product #PA5-18509). U251 cells were permeabilized with 0.15% Triton. Cells were incubated with 10 µg/mL of primary antibody for one hour followed by an Alexa Fluor 488 secondary antibody at a concentration of 2 µg/mL. Nuclear staining can be seen as shown above. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (2 µg/mL).

CK1 delta Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with CK1 delta siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CK1 delta Polyclonal Antibody (Product # PA5-18509). {KD}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DREERLRHSRNPATR) corresponding to the internal amino acids of CSNK1D

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10986846

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

Target Information

This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 1; casein kinase 1, delta; Casein kinase I isoform delta; CK1d; CK1delta; CKI-delta; CKId; HGNC:2452; Tau-protein kinase CSNK1D

Gene Aliases: ASPS; CKIdelta; CSNK1D; FASPS2; HCKID

UniProt ID: (Human) P48730

Entrez Gene ID: (Human) 1453

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase