Invitrogen

CK1 delta Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (11) CK1 delta antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (5)
Advanced Verification (1)

FIGURE: 1 / 6

CK1 delta Antibody (PA5-18509) in WB

Western blot was performed using Anti-CK1 delta Polyclonal Antibody (Product # PA5-18509) and a 49 kDa band corresponding to CK1 delta was observed across cell lines tested. Whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), MCF7 (Lane 2), HEK-293 (Lane 3) and HeLa (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary... View More

Product Image Gallery

CK1 delta Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CK1 delta was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CK1 delta Polyclonal Antibody (Product # PA5-18509) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32814), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear, cytoplasmic (golgi, ER) and cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CK1 delta Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with CK1 delta siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CK1 delta Polyclonal Antibody (Product # PA5-18509). {KD}

Product Details

PA5-18509

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (DREERLRHSRNPATR) corresponding to the internal amino acids of CSNK1D

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10986846

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

Target Information

CSNK1D (CK1 delta), a serine/threonine kinase, plays a critical role in Wnt/beta-catenin signaling pathway. This pathway is important in both development and cancer. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for CK1 delta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: casein kinase 1; casein kinase 1, delta; Casein kinase I isoform delta; CK1d; CK1delta; CKI-delta; CKId; HGNC:2452; Tau-protein kinase CSNK1D

Gene Aliases: ASPS; CKIdelta; CSNK1D; FASPS2; HCKID

UniProt ID: (Human) P48730

Entrez Gene ID: (Human) 1453

Molecular Function: non-receptor serine/threonine protein kinase