Invitrogen

CK1 delta Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (11) CK1 delta antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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CK1 delta Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CK1 delta was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CK1 delta Polyclonal Antibody (Product # PA5-18509) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32814), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear, cytoplasmic (golgi, ER) and cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CK1 delta Antibody in Western Blot (WB) — Western blot was performed using Anti-CK1 delta Polyclonal Antibody (Product # PA5-18509) and a 49 kDa band corresponding to CK1 delta was observed across cell lines tested. Whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), MCF7 (Lane 2), HEK-293 (Lane 3) and HeLa (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.75 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CK1 delta Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with CK1 delta siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CK1 delta Polyclonal Antibody (Product # PA5-18509). {KD}

Product Details

PA5-18509

Applications	Tested Dilution	Publications
Western Blot (WB)	0.3-1 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (DREERLRHSRNPATR) corresponding to the internal amino acids of CSNK1D View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10986846

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

Target Information

This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 1; casein kinase 1, delta; Casein kinase I isoform delta; CK1d; CK1delta; CKI-delta; CKId; HGNC:2452; Tau-protein kinase CSNK1D

Gene Aliases: ASPS; CKIdelta; CSNK1D; FASPS2; HCKID

UniProt ID: (Human) P48730

Entrez Gene ID: (Human) 1453

Molecular Function: non-receptor serine/threonine protein kinase