Invitrogen

CK1 delta Polyclonal Antibody

Product Image Gallery

CK1 delta Antibody in Western Blot (WB) — Western Blot analysis of precipitated CK1 delta from Hela whole cell lysate using a CK1 delta polyclonal antibody (Product # PA5-97636). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG (1µg). Lane 2: Hela whole cell lysate (500µg). Lane 3: Hela whole cell lysate (10µg).

CK1 delta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CK1 delta in paraffin embedded human lymph node tissue using a CK1 delta polyclonal antibody (Product # PA5-97636) at a dilution of 1:1000. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CK1 delta Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CK1 delta in HepG2 cells using a CK1 delta polyclonal antibody (Product # PA5-97636) at a dilution of 1:333. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000-1:5000

Immunohistochemistry (Paraffin) (IHC (P))

1:1000-1:2000

Immunocytochemistry (ICC)

1:200-1:500

Immunofluorescence (IF)

1:200-1:500

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

1:200-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human Casein kinase I isoform delta protein (152-398AA)

Conjugate

Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2812251

Target Information

This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: casein kinase 1; casein kinase 1, delta; Casein kinase I isoform delta; CK1d; CK1delta; CKI-delta; CKId; HGNC:2452; Tau-protein kinase CSNK1D

Gene Aliases: 1200006A05Rik; AA409348; ASPS; CKIdelta; CSNK1D; D930010H05Rik; FASPS2; HCKID

UniProt ID: (Human) P48730, (Rat) Q06486, (Mouse) Q9DC28

Entrez Gene ID: (Human) 1453, (Rat) 64462, (Mouse) 104318

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase