Invitrogen

CK1 delta Polyclonal Antibody

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CK1 delta Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CK1 delta in HepG2 cells using a CK1 delta polyclonal antibody (Product # PA5-97636) at a dilution of 1:333. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

CK1 delta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CK1 delta in paraffin embedded human skeletal muscle tissue using a CK1 delta polyclonal antibody (Product # PA5-97636) at a dilution of 1:1000. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CK1 delta Antibody in Western Blot (WB) — Western Blot analysis of CK1 delta using a CK1 delta Polyclonal antibody (Product # PA5-97636) at a concentration of 5 µg/mL. Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, Rat brain tissue, Mouse liver tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 48 kDa.

Product Details

PA5-97636

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000-1:2,000	-
Immunocytochemistry (ICC/IF)	1:200-1:500	-
ELISA (ELISA)	Assay-Dependent	-
Immunoprecipitation (IP)	1:200-1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Casein kinase I isoform delta protein (152-398AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	5 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812251

Target Information

This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 1; casein kinase 1, delta; Casein kinase I isoform delta; CK1d; CK1delta; CKI-delta; CKId; HGNC:2452; Tau-protein kinase CSNK1D

Gene Aliases: 1200006A05Rik; AA409348; ASPS; CKIdelta; CSNK1D; D930010H05Rik; FASPS2; HCKID

UniProt ID: (Human) P48730, (Rat) Q06486, (Mouse) Q9DC28

Entrez Gene ID: (Human) 1453, (Rat) 64462, (Mouse) 104318

Molecular Function: non-receptor serine/threonine protein kinase