Proteintech

CKMT1A Polyclonal Antibody

CKMT1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CKMT1A Antibody (15346-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CKMT1A Antibody in Immunoprecipitation (IP)

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Product Details

15346-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CKMT1A Fusion Protein Ag7583 (41-417 aa encoded by BC001926)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SERRRLYPP SAEYPDLRKH NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH PFIKTVGMVA GDEETYEVFA DLFDPVIQER HNGYDPRTMK HTTDLDASKI RSGYFDERYV LSSRVRTGRS IRGLSLPPAC TRAERREVER VVVDALSGLK GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG MARDWPDARG IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE RGWEFMWNER LGYILTCPSN LGTGLRAGVH IKLPLLSKDS RFPKILENLR LQKRGTGGVD TAATGGVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR LERGQDIRIP TPVIHTKH (41-417 aa encoded by BC001926 )

Target Information

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Zeb1/MtCK1 metabolic axis controls osteoclast activation and skeletal remodeling.

The EMBO journal

Zhu L,Tang Y,Li XY,Kerk SA,Lyssiotis CA,Feng W,Sun X,Hespe GE,Wang Z,Stemmler MP,Brabletz S,Brabletz T,Keller ET,Ma J,Cho JS,Yang J,Weiss SJ

Mon Apr 03 00:00:00 UTC 2023

A Zeb1/MtCK1 metabolic axis controls osteoclast activation and skeletal remodeling.

The EMBO journal

Zhu L,Tang Y,Li XY,Kerk SA,Lyssiotis CA,Feng W,Sun X,Hespe GE,Wang Z,Stemmler MP,Brabletz S,Brabletz T,Keller ET,Ma J,Cho JS,Yang J,Weiss SJ

Mon Apr 03 00:00:00 UTC 2023

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis.

Molecular oncology

Chen T,Zhang X,Ding X,Feng J,Zhang X,Xie D,Wang X

Sat Apr 01 00:00:00 UTC 2023

Investigations into photoreceptor energy metabolism during experimental retinal detachment.

Frontiers in cellular neuroscience

Chidlow G,Chan WO,Wood JPM,Casson RJ

Tue Dec 06 00:00:00 UTC 2022

Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.

Nature communications

Wang LJ,Chen CP,Lee YS,Ng PS,Chang GD,Pao YH,Lo HF,Peng CH,Cheong ML,Chen H

Fri Mar 25 00:00:00 UTC 2022

Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.

Nature communications

Wang LJ,Chen CP,Lee YS,Ng PS,Chang GD,Pao YH,Lo HF,Peng CH,Cheong ML,Chen H

Fri Mar 25 00:00:00 UTC 2022

Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.

Nature communications

Wang LJ,Chen CP,Lee YS,Ng PS,Chang GD,Pao YH,Lo HF,Peng CH,Cheong ML,Chen H

Fri Mar 25 00:00:00 UTC 2022

Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation.

Respiratory research

Li M,Liu H,Li J,Guo S,Lv Y

Thu Jul 01 00:00:00 UTC 2021

Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation.

Respiratory research

Li M,Liu H,Li J,Guo S,Lv Y

Thu Jul 01 00:00:00 UTC 2021

Accumulation of Carbonyl Proteins in the Brain of Mouse Model for Methylglyoxal Detoxification Deficits.

Antioxidants (Basel, Switzerland)

Koike S,Toriumi K,Kasahara S,Kibune Y,Ishida YI,Dan T,Miyata T,Arai M,Ogasawara Y

Thu Apr 08 00:00:00 UTC 2021

Distribution and Activity of Mitochondrial Proteins in Vascular and Avascular Retinas: Implications for Retinal Metabolism.

Investigative ophthalmology & visual science

Chidlow G,Wood JPM,Sia PI,Casson RJ

Wed Jan 02 00:00:00 UTC 2019

Distribution and Activity of Mitochondrial Proteins in Vascular and Avascular Retinas: Implications for Retinal Metabolism.

Investigative ophthalmology & visual science

Chidlow G,Wood JPM,Sia PI,Casson RJ

Wed Jan 02 00:00:00 UTC 2019

Distribution and Activity of Mitochondrial Proteins in Vascular and Avascular Retinas: Implications for Retinal Metabolism.

Investigative ophthalmology & visual science

Chidlow G,Wood JPM,Sia PI,Casson RJ

Wed Jan 02 00:00:00 UTC 2019

Distribution and Activity of Mitochondrial Proteins in Vascular and Avascular Retinas: Implications for Retinal Metabolism.

Investigative ophthalmology & visual science

Chidlow G,Wood JPM,Sia PI,Casson RJ

Wed Jan 02 00:00:00 UTC 2019

Distribution and Activity of Mitochondrial Proteins in Vascular and Avascular Retinas: Implications for Retinal Metabolism.

Investigative ophthalmology & visual science

Chidlow G,Wood JPM,Sia PI,Casson RJ

Wed Jan 02 00:00:00 UTC 2019

Distribution and Activity of Mitochondrial Proteins in Vascular and Avascular Retinas: Implications for Retinal Metabolism.

Investigative ophthalmology & visual science

Chidlow G,Wood JPM,Sia PI,Casson RJ

Wed Jan 02 00:00:00 UTC 2019

Investigations Into Bioenergetic Neuroprotection of Cone Photoreceptors: Relevance to Retinitis Pigmentosa.

Frontiers in neuroscience

Narayan DS,Chidlow G,Wood JPM,Casson RJ

Mon Sep 28 00:00:00 UTC 2020

Creatine is Neuroprotective to Retinal Neurons In Vitro But Not In Vivo.

Investigative ophthalmology & visual science

Sia PI,Wood JPM,Chidlow G,Casson R

Tue Oct 01 00:00:00 UTC 2019

Creatine is Neuroprotective to Retinal Neurons In Vitro But Not In Vivo.

Investigative ophthalmology & visual science

Sia PI,Wood JPM,Chidlow G,Casson R

Tue Oct 01 00:00:00 UTC 2019

Creatine is Neuroprotective to Retinal Neurons In Vitro But Not In Vivo.

Investigative ophthalmology & visual science

Sia PI,Wood JPM,Chidlow G,Casson R

Tue Oct 01 00:00:00 UTC 2019

Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.

Cell metabolism

Kurmi K,Hitosugi S,Yu J,Boakye-Agyeman F,Wiese EK,Larson TR,Dai Q,Machida YJ,Lou Z,Wang L,Boughey JC,Kaufmann SH,Goetz MP,Karnitz LM,Hitosugi T

Tue Dec 04 00:00:00 UTC 2018

Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.

Cell metabolism

Kurmi K,Hitosugi S,Yu J,Boakye-Agyeman F,Wiese EK,Larson TR,Dai Q,Machida YJ,Lou Z,Wang L,Boughey JC,Kaufmann SH,Goetz MP,Karnitz LM,Hitosugi T

Tue Dec 04 00:00:00 UTC 2018

Three human aminoacyl-tRNA synthetases have distinct sub-mitochondrial localizations that are unaffected by disease-associated mutations.

The Journal of biological chemistry

González-Serrano LE,Karim L,Pierre F,Schwenzer H,Rötig A,Munnich A,Sissler M

Fri Aug 31 00:00:00 UTC 2018

Three human aminoacyl-tRNA synthetases have distinct sub-mitochondrial localizations that are unaffected by disease-associated mutations.

The Journal of biological chemistry

González-Serrano LE,Karim L,Pierre F,Schwenzer H,Rötig A,Munnich A,Sissler M

Fri Aug 31 00:00:00 UTC 2018

Characterization of brown adipose tissue in the human perirenal depot.

Obesity (Silver Spring, Md.)

Svensson PA,Lindberg K,Hoffmann JM,Taube M,Pereira MJ,Mohsen-Kanson T,Hafner AL,Rizell M,Palming J,Dani C,Svensson MK

Fri Aug 01 00:00:00 UTC 2014

Characterization of brown adipose tissue in the human perirenal depot.

Obesity (Silver Spring, Md.)

Svensson PA,Lindberg K,Hoffmann JM,Taube M,Pereira MJ,Mohsen-Kanson T,Hafner AL,Rizell M,Palming J,Dani C,Svensson MK

Fri Aug 01 00:00:00 UTC 2014

Increased serum mitochondrial creatine kinase activity as a risk for hepatocarcinogenesis in chronic hepatitis C patients.

International journal of cancer

Enooku K,Nakagawa H,Soroida Y,Ohkawa R,Kageyama Y,Uranbileg B,Watanabe N,Tateishi R,Yoshida H,Koike K,Yatomi Y,Ikeda H

Fri Aug 15 00:00:00 UTC 2014

Increased serum mitochondrial creatine kinase activity as a risk for hepatocarcinogenesis in chronic hepatitis C patients.

International journal of cancer

Enooku K,Nakagawa H,Soroida Y,Ohkawa R,Kageyama Y,Uranbileg B,Watanabe N,Tateishi R,Yoshida H,Koike K,Yatomi Y,Ikeda H

Fri Aug 15 00:00:00 UTC 2014

Reconstitution of CKMT1 expression fails to rescue cells from mitochondrial membrane potential dissipation: implications for controlling RNAi experiments.

Biochimica et biophysica acta

Datler C,Grimm S

Sun Dec 01 00:00:00 UTC 2013

Reconstitution of CKMT1 expression fails to rescue cells from mitochondrial membrane potential dissipation: implications for controlling RNAi experiments.

Biochimica et biophysica acta

Datler C,Grimm S

Sun Dec 01 00:00:00 UTC 2013

Gene expression in human brown adipose tissue.

International journal of molecular medicine

Svensson PA,Jernås M,Sjöholm K,Hoffmann JM,Nilsson BE,Hansson M,Carlsson LM

Tue Feb 01 00:00:00 UTC 2011

Gene expression in human brown adipose tissue.

International journal of molecular medicine

Svensson PA,Jernås M,Sjöholm K,Hoffmann JM,Nilsson BE,Hansson M,Carlsson LM

Tue Feb 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Acidic-type mitochondrial creatine kinase; Creatine kinase U-type, mitochondrial; creatine kinase, mitochondrial 1 (ubiquitous); creatine kinase, mitochondrial 1, ubiquitous; Mia-CK; U-MtCK; Ubiquitous mitochondrial creatine kinase

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Gene Aliases: CKMT; CKMT1; CKMT1A; CKMT1B; mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK; UbCKmit

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UniProt ID: (Human) P12532, (Mouse) P30275

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Entrez Gene ID: (Human) 548596, (Mouse) 12716, (Rat) 29593

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Function(s)

creatine kinase activity ATP binding nucleotide binding catalytic activity protein binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups amino acid kinase

Process(es)

creatine metabolic process phosphorylation negative regulation of protein binding negative regulation of apoptotic process phosphocreatine biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CKMT1A Polyclonal Antibody

CKMT1A Antibody (15346-1-AP) in IHC (P)

Product Details

15346-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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