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Proteintech
KAP1 Monoclonal Antibody (1B9G12), CoraLite® Plus 647
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
CL64766630100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
1B9G12
Conjugate
CoraLite® Plus 647
CoraLite® Plus 647
CoraLite® Plus 647
Excitation/Emission Max
654/674 nm
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.5% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Wet ice
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; transcriptional intermediary factor 1-beta; tripartite motif; unnamed protein product
Gene Aliases: KAP1; PPP1R157; RNF96; TF1B; TIF1B; TIF1beta; WT7
UniProt ID: (Human) Q13263
Entrez Gene ID: (Human) 10155
DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
transcription corepressor activity
RNA binding
protein kinase activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
SUMO transferase activity
ubiquitin protein ligase binding
Krueppel-associated box domain binding
ubiquitin protein ligase activity
ubiquitin-like protein ligase activity
SUMO ligase activity
chromo shadow domain binding
chromatin-protein adaptor activity
promoter-specific chromatin binding
epithelial to mesenchymal transition
DNA repair
chromatin organization
methylation-dependent chromatin silencing
cellular response to DNA damage stimulus
protein ubiquitination
protein sumoylation
positive regulation of type I interferon production
positive regulation of protein import into nucleus
negative regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation by host of viral release from host cell
innate immune response
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
antiviral innate immune response
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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