Immunofluorescent analysis of CMPK1 showing staining in the cytoplasm and nucleus of HCT 116 cells. HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CMPK1 polyclonal antibody (Product # PA5-28268) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 189 of Human CMPK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28268 targets CMPK1 in WB applications and shows reactivity with Human and mouse samples.
The PA5-28268 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 189 of Human CMPK1.
Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 188.8.131.52) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.