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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
0.04-0.4 µg/mL | - |
Immunohistochemistry (IHC) |
1:20-1:50 | - |
Immunocytochemistry (ICC/IF) |
0.25-2 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein corresponding to Human CNOT1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 40% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2639954 |
Immunogen sequence: ALLQINTSWH TLRHELISTL MPIFLGNHPN SAIILHYAWH GQGQSPSIRQ LIMHAMAEWY MRGEQYDQAK LSRILDVAQD LKALSMLLNG T
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adrenal gland protein AD-005; CCR4-associated factor 1; CCR4-NOT transcription complex subunit 1; CCR4-NOT transcription complex, subunit 1; Negative regulator of transcription subunit 1 homolog; NOT1 (negative regulator of transcription 1, yeast) homolog; NOT1H
Gene Aliases: AD-005; CDC39; CNOT1; KIAA1007; NOT1; NOT1H
UniProt ID: (Human) A5YKK6
Entrez Gene ID: (Human) 23019
Molecular Function:
mRNA polyadenylation factor
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