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Invitrogen
CNOT4 Polyclonal Antibody
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FIGURE: 1 / 2
CNOT4 Antibody (PA5-101501) in ICC/IF
Immunocytochemistry analysis of CNOT4 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CNOT4 polyclonal antibody (Product # PA5-101501) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CNOT4(Accession O95628), corresponding to amino acid residues E62-F112.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CNOT4.
Target Information
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal.Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CCR4-associated factor 4; CCR4-NOT transcription complex subunit 4; E3 ubiquitin-protein ligase CNOT4; general negative regulator of transcription subunit 4; NLS-containing variant; negative regulator of transcription 4; NOT4 (negative regulator of transcription 4, yeast) homolog; Potential transcriptional repressor NOT4Hp; RING-type E3 ubiquitin transferase CNOT4; transcriptional repressor NOT4Hp; unnamed protein product
Gene Aliases: CLONE243; CNOT4; NOT4; NOT4H; Not4hp
UniProt ID: (Human) O95628, (Mouse) Q8BT14
Entrez Gene ID: (Human) 4850, (Rat) 312227, (Mouse) 53621
nucleic acid binding
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
metal ion binding
ubiquitin protein ligase activity
nucleotide binding
poly(A) RNA binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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