Invitrogen

COL14A1 Polyclonal Antibody

Product Image Gallery

COL14A1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of COL14A1 in HeLa cells. Samples were probed using a COL14A1 polyclonal antibody (Product # PA5-49916).

COL14A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of COL14A1 in paraffin-embedded human heart tissue. Samples were probed using a COL14A1 polyclonal antibody (Product # PA5-49916).

COL14A1 Antibody in Immunohistochemistry (IHC) — Fig. 7 Comparison of proteomic signatures of pulmonary fibrosis in conditional Nedd4-2 -/- mice and patients with IPF. a , b Heatmap of differentially regulated matrisome annotated proteins in lungs from conditional Nedd4-2 -/- mice ( n = 11) and control mice ( n = 13) induced with doxycycline for 3 months ( a ), and in lung tissues from IPF patients and controls ( n = 11/group) ( b ). c Venn-diagram showing proportion of unique and common differentially regulated proteins in lungs of conditional Nedd4-2 - /- mice and IPF patients. d Summary of differentially regulated matrisome proteins found in conditional Nedd4-2 - /- mice and human IPF samples. e Micrographs of representative lung sections from conditional Nedd4-2 - /- mice and littermate controls stained with anti-Tnc, anti-Col14a1, and anti-Serpinh1 antibodies ( n = 6/group). Scale bars, 100 um.

Product Details

PA5-49916

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-
Immunocytochemistry (ICC/IF)	1:100-1:200	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthesized peptide derived from internal of human Collagen XIV alpha1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2635369

Product Specific Information

The antibody detects endogenous levels of total COL14A1 protein.

Target Information

Type XIV collagen is a fibril-associated collagen with an interrupted triple helix. It interacts with the fibril surface and regulates fibrillogenesis (Ansorge et al., 2009 [PubMed 19136672]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulators of Collagen Fibrillogenesis during Molar Development in the Mouse.

Frontiers in physiology

Zvackova I,Matalova E,Lesot H

Published figure using COL14A1 polyclonal antibody (Product # PA5-49916) in Immunohistochemistry

Wed Sep 30 00:00:00 EDT 2020

Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice.

Nature communications

Duerr J,Leitz DHW,Szczygiel M,Dvornikov D,Fraumann SG,Kreutz C,Zadora PK,Seyhan Agircan A,Konietzke P,Engelmann TA,Hegermann J,Mulugeta S,Kawabe H,Knudsen L,Ochs M,Rotin D,Muley T,Kreuter M,Herth FJF,Wielpütz MO,Beers MF,Klingmüller U,Mall MA

PA5-49916 was used in Immunohistochemistry to show that conditional deletion of the ubiquitin ligase Nedd4-2 (Nedd4l) in lung epithelial cells in adult mice produces chronic lung disease sharing key features with idiopathic pulmonary fibrosis (IPF) including progressive fibrosis and bronchiolization with increased expression of Muc5b in peripheral airways, honeycombing and characteristic alterations in the lung proteome.

Fri Apr 24 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: col14a1; Collagen alpha-1(XIV) chain; collagen, type XIV, alpha 1; procollagen, type XIV, alpha 1; Undulin; undulin (fibronectin-tenascin-related)

Gene Aliases: 5730412L22Rik; AW108078; COL14A1; UND

UniProt ID: (Human) Q05707, (Mouse) Q80X19

Entrez Gene ID: (Human) 7373, (Mouse) 12818

Molecular Function: extracellular matrix structural protein