Invitrogen

COL1A1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

9 References

View all (7) COL1A1 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

COL1A1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of COL1A1 was performed using 70% confluent log phase SK-N-SH and HT-29 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with COL1A1 Polyclonal Antibody (Product # PA5-29569) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic localization. Panel (a-d) shows representative SK-N-SH control cell, whereas Panel (e-h) represent SK-N-SH with PTI treatment. Similarly, panel (i-l) represent HT-29 control cells and Panel (m-p) represent HT-29 with PTI treatment. The images were captured at 60 magnification.

COL1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — COL1A1 Polyclonal Antibody detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COL1A1 Polyclonal Antibody (Product # PA5-29569) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

COL1A1 Antibody in Western Blot (WB) — Western blot was performed using Anti-COL1A1 Polyclonal Antibody (Product # PA5-29569) and 160 kDa and 120kDa band corresponding to COL1A1 isoforms were observed in SK-N-SH, in comparison to HT-29. Whole cell lysate (30 µg lysate) of SK-N-SH (Lane 1), SK-N-SH with PTI treatment (Lane 2), HT-29 (Lane 3) and HT-29 with PTI treatment (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

COL1A1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of COL1A1 was performed in SK-N-AS whole cell extracts using 5 µg of COL1A1 Polyclonal Antibody (Product # PA5-29569). Samples were transferred to a membrane and probed with COL1A1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

COL1A1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-COL1A1 Polyclonal Antibody (Product # PA5-29569), shows detection in only SK-N-SH and upregulate upon PTI treatments in comparison to HT-29 with and without PTI treatment. {RE}

Product Details

PA5-29569

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	View 1 publication 1 publication
Immunoprecipitation (IP)	1:100-1:500	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 219 and 478 of Human COL1A1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547045

Product Specific Information

Recommended positive controls: SK-N-SH, SK-N-AS, NIH3T3.

Predicted reactivity: Mouse (96%), Rat (94%), Xenopus laevis (83%), Dog (98%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is the pro-alpha1 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deficient Endoplasmic Reticulum Acetyl-CoA Import in Pancreatic Acinar Cells Leads to Chronic Pancreatitis.

Cellular and molecular gastroenterology and hepatology

Cooley MM,Thomas DDH,Deans K,Peng Y,Lugea A,Pandol SJ,Puglielli L,Groblewski GE

PA5-29569 was used in Immunocytochemistry to examine the role of the ER acetyl-CoA transporter AT-1 on pancreatic homeostasis.

Mon Dec 20 00:00:00 EST 2021

Impaired non-canonical transforming growth factor-β signalling prevents profibrotic phenotypes in cultured peptidylarginine deiminase 4-deficient murine cardiac fibroblasts.

Journal of cellular and molecular medicine

Akboua H,Eghbalzadeh K,Keser U,Wahlers T,Paunel-Görgülü A

Published figure using COL1A1 polyclonal antibody (Product # PA5-29569) in Western Blot

Fri Oct 01 00:00:00 EDT 2021

Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.

RNA biology

Gan S,Su C,Ma J,Liu M,Cui X,Xin L,Ren Y,Gao X,Ge L,Wei M,Yang J

PA5-29569 was used in Western Blot to suggest that the translational efficiency of Tudor-SN is regulated by the mTORC1 pathway in myocardia and that Tudor-SN is involved in mTORC1-mediated regulation of cardiomyocytic proliferation and cardiac development.

Tue Jun 01 00:00:00 EDT 2021

An Injectable Fibrin Scaffold Rich in Growth Factors for Skin Repair.

BioMed research international

Shao Z,Lyu C,Teng L,Xie X,Sun J,Zou D,Lu J

PA5-29569 was used in Immunohistochemistry to introduce some characteristics of IFS.

Tue May 25 00:00:00 EDT 2021

Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart.

International journal of molecular sciences

Jelinkova S,Sleiman Y,Fojtík P,Aimond F,Finan A,Hugon G,Scheuermann V,Beckerová D,Cazorla O,Vincenti M,Amedro P,Richard S,Jaros J,Dvorak P,Lacampagne A,Carnac G,Rotrekl V,Meli AC

PA5-29569 was used in Western Blot to identify the early recruitment of CVPCs in dystrophic hearts and their fast depletion with ageing. This latter depletion may participate in the fibrosis development and the acceleration onset of the cardiomyopathy.

Mon May 10 00:00:00 EDT 2021

Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart.

International journal of molecular sciences

Jelinkova S,Sleiman Y,Fojtík P,Aimond F,Finan A,Hugon G,Scheuermann V,Beckerová D,Cazorla O,Vincenti M,Amedro P,Richard S,Jaros J,Dvorak P,Lacampagne A,Carnac G,Rotrekl V,Meli AC

Mon May 10 00:00:00 EDT 2021

Small molecule inhibitor of HSP47 prevents pro-fibrotic mechanisms of fibroblasts in vitro.

Biochemical and biophysical research communications

Miyamura T,Sakamoto N,Kakugawa T,Taniguchi H,Akiyama Y,Okuno D,Moriyama S,Hara A,Kido T,Ishimoto H,Yamaguchi H,Miyazaki T,Obase Y,Ishimatsu Y,Tanaka Y,Mukae H

PA5-29569 was used in Western Blot to demonstrate that HSP47 is a potential therapeutic target for pulmonary fibrosis.

Thu Sep 24 00:00:00 EDT 2020

FOXM1 is a critical driver of lung fibroblast activation and fibrogenesis.

The Journal of clinical investigation

Penke LR,Speth JM,Dommeti VL,White ES,Bergin IL,Peters-Golden M

PA5-29569 was used in Western Blotting to identify FOXM1 as a driver of lung fibroblast activation and underscore the therapeutic potential of targeting FOXM1 for pulmonary fibrosis.

Fri Jun 01 00:00:00 EDT 2018

Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor γ in MC3T3-E1 cells.

Biochemical and biophysical research communications

Qu B,Ma Y,Yan M,Gong K,Liang F,Deng S,Jiang K,Ma Z,Pan X

PA5-29569 was used in western blot to investigate the molecular mechanism of Sirt1 in osteogenic differentiation

Fri Sep 09 00:00:00 EDT 2016

MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor β/δ-dependent manner.

Biochemical and biophysical research communications

Gong K,Qu B,Liao D,Liu D,Wang C,Zhou J,Pan X

PA5-29569 was used in western blot to elucidate mechanisms by which miR-132 regulates osteogenic differentiation

Fri Sep 09 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; COL1A; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; Collagen1A1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen

Gene Aliases: Col1a-1; COL1A1; Cola-1; Cola1; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4

UniProt ID: (Human) Q15201, (Rat) P02454, (Mouse) P11087

Entrez Gene ID: (Human) 1277, (Rat) 29393, (Mouse) 12842

Molecular Function: extracellular matrix structural protein