Invitrogen

COL1A1 Propeptide Polyclonal Antibody

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COL1A1 Propeptide Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunofluorescent analysis of Collagen I alpha 1 propeptide in formaldehyde-fixed, paraffin-embedded fibrotic mouse lung tissue using a Collagen I alpha 1 propeptide polyclonal antibody (Product # PA5-35379) showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized. Prior to staining, antigen retrieval was performed by incubating tissues for 20 min at 90°C in 10mM sodium citrate (pH 6.0)/0.1 % Tween-20.

COL1A1 Propeptide Antibody in Western Blot (WB) — Western blot analysis of Collagen I alpha 1 propeptide in rat lung lysate using a Collagen I alpha 1 propeptide polyclonal antibody (Product # PA5-35379). Results show a band at ~180kDa.

Product Details

PA5-35379

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (IHC)	1:100	-
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to amino acid residues specific to human collagen 1, alpha 1 propeptide conjugated to KLH
Conjugate	Unconjugated
Form	Liquid
Concentration	~0.15 mg/mL
Purification	Affinity chromatography
Storage buffer	10mM HEPES, pH 7.5, with 150mM NaCl, 100µg/mL BSA, 50% glycerol
Contains	no preservative
Storage conditions	-20°C
RRID	AB_2552689

Product Specific Information

This antibody is predicted to react with most mammals, birds, amphibians and fish based on 100% sequence homology.

This antibody contains enough material to conduct 10 mini-Western blots.

Target Information

Type Icollagenis a triple helix comprised of two alpha-1 chains and one alpha-2 chain. Type I collagen is a member of group I collagen (fibril-formingcollagen)found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A1 gene encoding the alpha-1 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The mononuclear phagocyte system contributes to fibrosis in post-transplant obliterans bronchiolitis.

The European respiratory journal

Di Campli MP,Azouz A,Assabban A,Scaillet J,Splittgerber M,Van Keymeulen A,Libert F,Remmelink M,Le Moine A,Lemaitre P,Goriely S

PA5-35379 was used in Immunohistochemistry (Frozen) to conclude that therapies targeting the mononuclear phagocyte system could improve long-term outcomes after lung transplantation.

Mon Mar 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; COL1A; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen

Gene Aliases: Col1a-1; COL1A1; Cola-1; Cola1; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4

UniProt ID: (Human) P02452, (Rat) P02454, (Mouse) P11087

Entrez Gene ID: (Human) 1277, (Rat) 29393, (Mouse) 12842

Molecular Function: extracellular matrix structural protein