Invitrogen

COL1A1 Propeptide Polyclonal Antibody

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COL1A1 Propeptide Antibody in Western Blot (WB) — Western blot analysis of Collagen I alpha 1 propeptide in rat lung lysate using a Collagen I alpha 1 propeptide polyclonal antibody (Product # PA5-35379). Results show a band at ~180kDa.

COL1A1 Propeptide Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunofluorescent analysis of Collagen I alpha 1 propeptide in formaldehyde-fixed, paraffin-embedded fibrotic mouse lung tissue using a Collagen I alpha 1 propeptide polyclonal antibody (Product # PA5-35379) showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized. Prior to staining, antigen retrieval was performed by incubating tissues for 20 min at 90°C in 10mM sodium citrate (pH 6.0)/0.1 % Tween-20.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

Immunohistochemistry (IHC)

1:100

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acid residues specific to human collagen 1, alpha 1 propeptide conjugated to KLH

Conjugate

Unconjugated

Form

Liquid

Concentration

~0.15 mg/mL

Purification

Affinity chromatography

Storage buffer

10mM HEPES, pH 7.5, with 150mM NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2552689

Product Specific Information

This antibody is predicted to react with most mammals, birds, amphibians and fish based on 100% sequence homology.

This antibody contains enough material to conduct 10 mini-Western blots.

Target Information

Type Icollagenis a triple helix comprised of two alpha-1 chains and one alpha-2 chain. Type I collagen is a member of group I collagen (fibril-formingcollagen)found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A1 gene encoding the alpha-1 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha-1 type 1 collagen; Alpha-1 type I collagen; alpha1(I) procollagen; COL1A; Collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; Type I Collagen; type I proalpha 1; type I procollagen alpha 1 chain; type I-alpha 1 collagen

Gene Aliases: Col1a-1; COL1A1; Cola-1; Cola1; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4

UniProt ID: (Human) P02452, (Rat) P02454, (Mouse) P11087

Entrez Gene ID: (Human) 1277, (Rat) 29393, (Mouse) 12842

Function(s)