Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
COL1A2 Antibody (PA5-120479) in WB
Western blot analysis of COL1A2 in extracts of various cell lines. Samples were incubated with COL1A2 Polyclonal antibody (Product # PA5-120479) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-120479
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1161-1260 of human Collagen I/COL1A2 (NP_0000802)
Conjugate
Form
Liquid
Concentration
0.64 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914051
Product Specific Information
Positive test controls include: Rat skeletal muscle, Rat skin. The target is usually found in the following locations: Secreted, extracellular matrix, extracellular space.^M
^M
Immunogen sequence: RTCRDLRLSH PEWSSGYYWI DPNQGCTMDA IKVYCDFSTG ETCIRAQPEN IPAKNWYRSS KDKKHVWLGE TINAGSQFEY NVEGVTSKEM ATQLAFMRLL
Target Information
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-2 type I collagen; COL1; Collagen alpha-2(I) chain; collagen COL1A2; Collagen I; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-2 chain; collagen, type I, alpha 2; osteogenesis imperfecta; procollagen, type I, alpha 2; type I procollagen
Gene Aliases: AA960264; AI325291; Col1a-2; COL1A2; Cola-2; Cola2; OI4; oim
UniProt ID: (Human) P08123, (Mouse) Q01149, (Rat) P02466
Entrez Gene ID: (Human) 1278, (Mouse) 12843, (Rat) 84352
skeletal system development
blood vessel development
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
blood coagulation
regulation of blood pressure
platelet activation
extracellular matrix organization
collagen fibril organization
collagen catabolic process
odontogenesis
skin morphogenesis
regulation of immune response
leukocyte migration
protein heterotrimerization
cellular response to amino acid stimulus
cellular response to retinoic acid
cellular response to organic substance
response to norepinephrine
cellular response to thyroid hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support