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FIGURE: 1 / 1
COLQ Antibody (PA5-109572) in WB
Western blot analysis of COLQ in extracts of various cell lines. Samples were incubated with COLQ Polyclonal antibody (Product # PA5-109572) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-109572
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 276-455 of human COLQ (NP_005668.2).
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2854983
Product Specific Information
Immunogen sequence: MGPKGERGFP GPPGRCLCGP TMNVNNPSYG ESVYGPSSPR VPVIFVVNNQ EELERLNTQN AIAFRRDQRS LYFKDSLGWL PIQLTPFYPV DYTADQHGTC GDGLLQPGEE CDDGNSDVGD DCIRCHRAYC GDGHRHEGVE DCDGSDFGYL TCETYLPGSY GDLQCTQYCY IDSTPCRYFT
Target Information
COLQ (collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase), also known as EAD, is a 455 amino acid protein that localizes to the end plate of skeletal muscle. COLQ anchors the catalytic subunits of asymmetric AChE (acetylcholinesterase) to the basal lamina at the neuromuscular junctions of vertebrates. Mutations of COLQ lead to congenital myasthenic syndromes which are rare autosomal recessive diseases characterized by general weakness increased by exertion, ophthalmoplegia and refractoriness to anticholinesterase drugs. Eight isoforms exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acetylcholinesterase collagenic tail peptide; Acetylcholinesterase-associated collagen; AChE Q subunit; collagen-like tail subunit of asymmetric acetylcholinesterase
Gene Aliases: A130034K24Rik; Colq
UniProt ID: (Mouse) O35348, (Rat) O35167
Entrez Gene ID: (Mouse) 382864, (Rat) 29755
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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