Proteintech

CORO1C Polyclonal Antibody

View all (7) Coronin 3 antibodies

Questions & Answers

Questions & Answers

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CORO1C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using CORO1C antibody 14749-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

CORO1C Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 14749-1-AP ( CORO1C antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CORO1C Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 14749-1-AP ( CORO1C antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CORO1C Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CORO1C Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CORO1C Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CORO1C Antibody in Western Blot (WB) — SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CORO1C Antibody in Western Blot (WB) — SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CORO1C Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 14749-1-AP (CORO1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CORO1C Antibody in Immunoprecipitation (IP) — IP result of anti-CORO1C (IP:14749-1-AP, 4ug; Detection:14749-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

Product Details

14749-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Artificial Control, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CORO1C Fusion Protein Ag6488 (1-306 aa encoded by BC002342) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.22 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MRRVVRQSK FRHVFGQAVK NDQCYDDIRV SRVTWDSSFC AVNPRFVAII IEASGGGAFL VLPLHKTGRI DKSYPTVCGH TGPVLDIDWC PHNDQVIASG SEDCTVMVWQ IPENGLTLSL TEPVVILEGH SKRVGIVAWH PTARNVLLSA GCDNAIIIWN VGTGEALINL DDMHSDMIYN VSWNRNGSLI CTASKDKKVR VIDPRKQEIV AEKEKAHEGA RPMRAIFLAD GNVFTTGFSR MSERQLALWN PKNMQEPIAL HEMDTSNGVL LPFYDPDTSI IYLCGKGDSS IRYFEITDES PYVHYLN (1-306 aa encoded by BC002342)

Target Information

Coronin 3 is associated with the aggression and metastasis of hepatocellular carcinoma. Coronins are a family of highly evolutionary conserved proteins reportedly involved in the regulation of actin cytoskeletal dynamics, although only coronin 3 has been shown to be related to cancer cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Novel Class of ER Membrane Proteins Regulates ER-Associated Endosome Fission.

Cell

Hoyer MJ,Chitwood PJ,Ebmeier CC,Striepen JF,Qi RZ,Old WM,Voeltz GK

Thu Sep 20 00:00:00 EDT 2018

Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice.

Human molecular genetics

Pellegrini L,Hauser DN,Li Y,Mamais A,Beilina A,Kumaran R,Wetzel A,Nixon-Abell J,Heaton G,Rudenko I,Alkaslasi M,Ivanina N,Melrose HL,Cookson MR,Harvey K

Sat Sep 15 00:00:00 EDT 2018

Knockdown of Coronin-1C disrupts Rac1 activation and impairs tumorigenic potential in hepatocellular carcinoma cells.

Oncology reports

Wang ZG,Jia MK,Cao H,Bian P,Fang XD

Fri Mar 01 00:00:00 EST 2013

Coronin-1C is a novel biomarker for hepatocellular carcinoma invasive progression identified by proteomics analysis and clinical validation.

Journal of experimental & clinical cancer research : CR

Wu L,Peng CW,Hou JX,Zhang YH,Chen C,Chen LD,Li Y

Wed Feb 24 00:00:00 EST 2010

Coronin-1C is a novel biomarker for hepatocellular carcinoma invasive progression identified by proteomics analysis and clinical validation.

Journal of experimental & clinical cancer research : CR

Wu L,Peng CW,Hou JX,Zhang YH,Chen C,Chen LD,Li Y

Wed Feb 24 00:00:00 EST 2010

Bioinformatics

Protein Aliases: coronin 1c; coronin 3; coronin, actin binding protein, 1C; coronin, actin-binding protein, 1C; Coronin-1C; Coronin-3; hCRNN4

Gene Aliases: AL022675; AW455561; AW548837; CORO1C; CRN2; CRNN4; HCRNN4; RGD1564490

UniProt ID: (Human) Q9ULV4, (Mouse) Q9WUM4

Entrez Gene ID: (Human) 23603, (Rat) 501841, (Mouse) 23790

Molecular Function: non-motor actin binding protein

neural crest cell migration regulation of protein phosphorylation negative regulation of protein phosphorylation phagocytosis signal transduction regulation of epithelial cell migration negative regulation of epithelial cell migration regulation of fibroblast migration actin cytoskeleton organization negative regulation of protein kinase activity by regulation of protein phosphorylation establishment of protein localization regulation of focal adhesion assembly negative regulation of focal adhesion assembly activation of GTPase activity regulation of substrate adhesion-dependent cell spreading negative regulation of substrate adhesion-dependent cell spreading regulation of ruffle assembly positive regulation of lamellipodium morphogenesis actin cortical patch assembly

Process(es)

protein binding Rac GTPase binding actin filament binding actin binding