Invitrogen

CPOX Polyclonal Antibody

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CPOX Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CPOX in HepG2 cells using a CPOX polyclonal antibody (Product # PA5-97613) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

CPOX Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CPOX in paraffin embedded human liver tissue using a CPOX polyclonal antibody (Product # PA5-97613) at a dilution of 1:300. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

CPOX Antibody in Western Blot (WB) — Western Blot analysis of CPOX using a CPOX Polyclonal antibody (Product # PA5-97613) at a concentration of 5.7 µg/mL. Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 51 kDa.

Product Details

PA5-97613

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial protein (19-149AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.825 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812228

Target Information

The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: coprogen oxidase; coproporphyrinogen-III oxidase, mitochondrial; coproporphyrinogenase; COX; Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial

Gene Aliases: CPO; CPOX; CPX; HCP

UniProt ID: (Human) P36551

Entrez Gene ID: (Human) 1371

Molecular Function: oxidase oxidoreductase metabolite interconversion enzyme