Immunofluorescent analysis of CPSF2 showing staining in the nucleus of NT2D1 cells. NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CPSF2 polyclonal antibody (Product # PA5-34862) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 92 and 317 of CPSF2 (Uniprot ID#Q9P2I0)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34862 targets CPSF2 in WB applications and shows reactivity with Human samples.
The PA5-34862 immunogen is recombinant fragment corresponding to a region within amino acids 92 and 317 of CPSF2 (Uniprot ID#Q9P2I0).
Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.