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Invitrogen
CRX Polyclonal Antibody
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FIGURE: 1 / 2
CRX Antibody (PA5-47897) in ICC/IF
Immunocytochemistry analysis of CRX in immersion fixed Y‚79 human retinoblastoma cell line. Samples were incubated in CRX polyclonal antibody (Product # PA5-47897) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm.
Product Details
PA5-47897
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human CRX Ala166-Leu299
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
The cone-rod homeobox-containing gene (CRX) encodes a transcription factor that coordinates the expression of several photoreceptor genes in the developing retina, including opsin and rhodopsin. Specifically, CRX binds the OTX motif (TAATCC/A) upstream from photoreceptor genes. The CRX gene is also expressed in the pinealocytes of the pineal gland and may regulate pineal circadian activity by controling the expression of melatonin synthesis genes. Furthermore, CRX(-) mice exhibit disruption of circadian rhythms. The human CRX gene maps to chromosome 19q13.3 within the region of the cone-rod dystrophy-2 locus (CORD2). Mutations in the CRX gene are implicated in the visual pathologies of CORD, Leber congenital amaurosis (LCA) and retinitis pigmentosa (RP). All characterized CRX gene mutations produce disease in heterozygotes although there is no known correlation between the pathologic phenotype and genetic mutation. Missense mutations of the CRX gene affect the homeobox domain, whereas frameshift mutations affect the OTX domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cone rod homeobox; Cone-rod homeobox protein; CORD 2; CRXL1; LCA 7; orthodenticle homeobox 3; OTX; OTX 3; photoreceptor-specific transcription factor
Gene Aliases: CORD2; CRD; CRX; LCA7; OTX3
UniProt ID: (Human) O43186
Entrez Gene ID: (Human) 1406
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
ligand-dependent nuclear receptor binding
leucine zipper domain binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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