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Agrisera
CRY1/Cryptochrome 1 Polyclonal Antibody
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FIGURE: 1 / 1
CRY1/Cryptochrome 1 Antibody (AS16-3933) in WB
15 µg of total protein from Arabidopsis thaliana etiolated/greening seedling extracted with exact buffer (0.125 M Tris, 1% SDS, 10% Glycerol, 0.05 M Sodium metabisulfite) and denatured at 70°C for 5 min. Samples and Prestained Protein were separated on 10% SDS-PAGE(1.5 mm, 10-well) and blotted 50 min to PVDF using semi-dry. Blots were blocked with 5% nonfat milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for overnight at 4°C with agitation in TBS-T. The antibody solu... View More
Product Details
AS16-3933
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CRY1.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Nicotiana tabacum
Target Information
Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATCRY1; BLU1; BLUE LIGHT PHOTORECEPTOR; BLUE LIGHT UNINHIBITED 1; CRYPTOCHROME 1 APOPROTEIN; ELONGATED HYPOCOTYL 4; HY4; OOP2; OUT OF PHASE 2; T3H13.14; T3H13_14
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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