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Invitrogen
CTCF Polyclonal Antibody
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FIGURE: 1 / 1
CTCF Antibody (PA5149569) in ChIP
ChIP Assay of CTCF in K562 cells. ChIP-Exo-Seq composite graph for CTCF polyclonal antibody (Product # PA5149569). Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site.
Product Details
PA5149569
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human CTCF
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: QNQTDGGEVV QDVNSSVQMV MMEQLDPTLL QMKTEVMEGT VAPEAEAAVD DTQIITLQVV NMEEQPINIG ELQLVQVPVP VTVPVATTSV EELQGAYENE VSKEGLAESE PMICHTLPLP EGFQVVKVGA NGEVETLEQG ELPPQEDP
Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 100%.
Target Information
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF
Gene Aliases: CTCF; MRD21
UniProt ID: (Human) P49711
Entrez Gene ID: (Human) 10664
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
zinc ion binding
chromatin insulator sequence binding
sequence-specific DNA binding
transcription regulatory region DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
regulation of gene expression by genetic imprinting
transcription from RNA polymerase II promoter
chromosome segregation
maintenance of DNA methylation
positive regulation of gene expression
covalent chromatin modification
nucleosome positioning
regulation of histone methylation
regulation of histone acetylation
regulation of gene expression, epigenetic
regulation of molecular function, epigenetic
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of centromeric sister chromatid cohesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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