Invitrogen

CUX1/Protein CASP Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (6) CUX1/Protein CASP antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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CUX1/Protein CASP Antibody in Western Blot (WB) — Western blot was performed using Anti-CUX1 Polyclonal Antibody (Product # PA5-36355) and 72kDa, 77kDa, 147kDa, 167kDa bands corresponding to CUX1 was observed across cell lines tested. Whole cell extracts (30ug) of K-562 (Lane 1), MCF7 (Lane 2), Raji (Lane 3), NIH/3T3 (Lane 4) and PANC-1 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CUX1/Protein CASP Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with CUX1 siRNA and reduction of signal was observed in Western Blot using CUX1 Polyclonal Antibody (Product # PA5-36355). {KD}

Product Details

Applications

Tested Dilution

Publications

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:500-1:1000

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids N-terminus of human CDP

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2553490

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 78 kDa.

Purity is >95% by SDS-PAGE.

Target Information

The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CCAAT displacement protein; CDP; CDP2; cut homolog; cut-like 1; cut-like homeobox 1; Cux/CDP homeoprotein; golgi integral membrane protein 6; Homeobox protein cut-like 1; Homeobox protein cux-1; Protein CASP; putative protein product of Nbla10317

Gene Aliases: CASP; CDP; CDP/Cut; CDP1; Cdpl1; Clox; COY1; CUTL1; CUX; Cux-1; Cux/CDP; CUX1; GOLIM6; Kiaa4047; Nbla10317; p100; p110; p200; p75

UniProt ID: (Human) Q13948, (Rat) P53565, (Mouse) P53564

Entrez Gene ID: (Human) 1523, (Rat) 116639, (Mouse) 13047

Molecular Function: helix-turn-helix transcription factor homeobox transcription factor nucleic acid binding transcription factor