Invitrogen

CUX1/Protein CASP Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (6) CUX1/Protein CASP antibodies

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Datasheet

Protocols

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Tech Support

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CUX1/Protein CASP Antibody in Western Blot (WB) — Western blot was performed using Anti-CUX1 Polyclonal Antibody (Product # PA5-36355) and 72kDa, 77kDa, 147kDa, 167kDa bands corresponding to CUX1 was observed across cell lines tested. Whole cell extracts (30ug) of K-562 (Lane 1), MCF7 (Lane 2), Raji (Lane 3), NIH/3T3 (Lane 4) and PANC-1 (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CUX1/Protein CASP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with CUX1 siRNA and reduction of signal was observed in Western Blot using CUX1 Polyclonal Antibody (Product # PA5-36355). {KD}

Product Details

PA5-36355

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to amino acids N-terminus of human CDP View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2553490

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 78 kDa.

Purity is >95% by SDS-PAGE.

Target Information

The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CCAAT displacement protein; CDP; CDP/Cux p110; CDP/Cux p200; CDP2; cut homolog; cut-like 1; cut-like homeobox 1; Cux/CDP homeoprotein; golgi integral membrane protein 6; Homeobox protein cut-like 1; Homeobox protein cux-1; Protein CASP; putative protein product of Nbla10317

Gene Aliases: CASP; CDP; CDP/Cut; CDP1; Cdpl1; Clox; COY1; CUTL1; CUX; Cux-1; Cux/CDP; CUX1; GOLIM6; Kiaa4047; Nbla10317; p100; p110; p200; p75

UniProt ID: (Human) Q13948, (Rat) P53565, (Mouse) P53564

Entrez Gene ID: (Human) 1523, (Rat) 116639, (Mouse) 13047

Molecular Function: homeodomain transcription factor