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Invitrogen
CXCL5 Polyclonal Antibody, Biotin, PeproTech®
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FIGURE: 1 / 3
CXCL5 Antibody (500-P91BT-1MG) in WB
Western Blot: To detect hENA-78 by Western Blot analysis CXCL5 Polyclonal Antibody, Biotin (Product # 500-P91BT-500UG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P91BT-1MG
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human ENA-78 (CXCL5) (5-78 a.a.)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
Product Specific Information
AA Sequence of recombinant protein: AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human ENA-78 (CXCL5). Anti-Human ENA-78 (CXCL5)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hENA-78 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human ENA-78 (500-P91) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hENA-78.
Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P91BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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