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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
2-4 µg/mL | - |
Immunocytochemistry (ICC/IF) |
5-10 µg/mL | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to residues D(32) K Q T Q K L L K V P K G S(45) of human CYLD. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.0 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS |
Contains |
0.05% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2261341 |
This antibody is predicted to react with rat based on sequence homology.
Suggested positive control: Hela whole cell extract.
CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cylindromatosis (turban tumor syndrome); Deubiquitinating enzyme CYLD; FLJ20180; FLJ31664; FLJ78684; probable ubiquitin carboxyl-terminal hydrolase CYLD; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; Ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; Ubiquitin-specific-processing protease CYLD
Gene Aliases: BRSS; CDMT; CYLD; CYLD1; CYLDI; EAC; HSPC057; KIAA0849; MFT; MFT1; SBS; TEM; USPL2
UniProt ID: (Human) Q9NQC7
Entrez Gene ID: (Human) 1540
Molecular Function:
ribosomal protein
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