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      CYLD Polyclonal Antibody

      See 4 other CYLD antibodies ›
      CYLD Polyclonal Antibody for Western Blot, IF and ICC

      Catalog#: PA1-41097 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA1-41097 100 µg
      375.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human
      Published species reactivity Human
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Synthetic peptide corresponding to residues D(32) K Q T Q K L L K V P K G S(45) of human CYLD.
      Conjugate Unconjugated
      Form Liquid
      Concentration 1.0 mg/ml
      Purification Protein G
      Storage buffer PBS
      Contains 0.05% sodium azide
      Storage Conditions Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
      Tested Applications Dilution *
      Immunocytochemistry (ICC) 5-10 µg/ml
      Immunofluorescence (IF) 5-10 µg/ml
      Western Blot (WB) 2-4 µg/ml

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Miscellaneous PubMed (MISC) See 1 publications below

      Product Specific Information

      This antibody is predicted to react with rat based on sequence homology.

      Suggested positive control: Hela whole cell extract.

      Background/Target Information

      CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Miscellaneous PubMed

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human 1:250 Phospho-¿Np63¿-responsive microRNAs contribute to the regulation of necroptosis in squamous cell carcinoma upon cisplatin exposure.
      PA1-41097 was used in western blot to report the effect of DeltaNp63alpha-responsive microRNAs on the necroptotic pathway in squamous cell carcinoma tumor cells responding to platinum chemotherapy       		Ratovitski EA FEBS letters (589:1352) 2015

      Bioinformatics

      Protein Aliases:

      CDMT; CYLD1; CYLDI; cylindromatosis (turban tumor syndrome); Deubiquitinating enzyme CYLD; EAC; FLJ20180; FLJ31664; FLJ78684; HSPC057; KIAA0849; MFT; MFT1; probable ubiquitin carboxyl-terminal hydrolase CYLD; SBS; TEM; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; Ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; Ubiquitin-specific-processing protease CYLD; USPL2

      Gene Aliases:

      BRSS; CDMT; CYLD; CYLD1; CYLDI; EAC; HSPC057; KIAA0849; MFT; MFT1; SBS; TEM; USPL2

      UniProt ID:

      (Human) Q9NQC7

      Entrez Gene ID:

      (Human) 1540

      Molecular Function:

      RNA binding protein nucleic acid binding ribosomal protein

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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