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Invitrogen
CYLD Polyclonal Antibody
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Product Details
PA5-18688
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (DSQQSKSKNPWYIDE) corresponding to the internal amino acids of Cyld (aa 361-375).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
CYLD is a 956 aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme CYLD; FLJ20180; FLJ31664; FLJ78684; probable ubiquitin carboxyl-terminal hydrolase CYLD; retinitis pigmentosa 1 homolog; Ubiquitin carboxyl-terminal hydrolase CYLD; Ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; Ubiquitin-specific-processing protease CYLD
Gene Aliases: 2010013M14Rik; 2900009M21Rik; C130039D01Rik; CDMT; Cyld; CYLD1; EAC; Kiaa0849; LRRGT00003; mKIAA0849; Rp1; Rp1h
UniProt ID: (Rat) Q66H62, (Mouse) Q80TQ2
Entrez Gene ID: (Rat) 312937, (Mouse) 74256
molecular_function
structural constituent of ribosome
thiol-dependent ubiquitin-specific protease activity
zinc ion binding
protein kinase binding
Lys63-specific deubiquitinase activity
proline-rich region binding
protein binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
metal ion binding
ribosomal protein
cytoplasmic translation
ubiquitin-dependent protein catabolic process
regulation of mitotic cell cycle
biological_process
Wnt signaling pathway
protein deubiquitination
negative regulation of NF-kappaB transcription factor activity
negative regulation of NF-kappaB import into nucleus
negative regulation of T cell differentiation
necroptotic process
regulation of microtubule cytoskeleton organization
protein K63-linked deubiquitination
negative regulation of canonical Wnt signaling pathway
ripoptosome assembly involved in necroptotic process
regulation of cilium assembly
positive regulation of extrinsic apoptotic signaling pathway
regulation of intrinsic apoptotic signaling pathway
proteolysis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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