PA1-341 detects cytochrome P450 (P450) 1A2 from human cells as well as recombinant human P450 1A2. This antibody detects, to a lesser extent, other P450 isoforms.
PA1-341 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~56 kDa protein representing recombinant human P450 1A2.
The PA1-341 immunogen is purified, His-tagged, full length human P450 1A2 fusion protein.
The cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CYP; CYPIA2; Cytochrome P(3)450; Cytochrome P450 1A2; Cytochrome P450 4; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; Cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; microsomal monooxygenase; P(3)450; P450; P450 4; P450 form 4; P450-P3; xenobiotic monooxygenase
Gene Aliases: CP12; CYP1A2; P3-450; P450(PA)
UniProt ID: (Human) P05177
Entrez Gene ID: (Human) 1544