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CYP20A1 Antibody (PA5-109611) in WB
Western blot analysis of CYP20A1 in extracts of various cell lines. Samples were incubated with CYP20A1 Polyclonal antibody (Product # PA5-109611) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-109611
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 183-462 of human CYP20A1 (NP_803882.1).
Conjugate
Form
Liquid
Concentration
0.63 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855022
Product Specific Information
Immunogen sequence: STFEDDQEVI RFQKNHGTVW SEIGKGFLDG SLDKNMTRKK QYEDALMQLE SVLRNIIKER KGRNFSQHIF IDSLVQGNLN DQQILEDSMI FSLASCIITA KLCTWAICFL TTSEEVQKKL YEEINQVFGN GPVTPEKIEQ LRYCQHVLCE TVRTAKLTPV SAQLQDIEGK IDRFIIPRET LVLYALGVVL QDPNTWPSPH KFDPDRFDDE LVMKTFSSLG FSGTQECPEL RFAYMVTTVL LSVLVKRLHL LSVEGQVIET KYELVTSSRE EAWITVSKRY
Target Information
CYP20A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D, E)T, suggesting that its substrate may carry its own oxygen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytochrome P450 20A1; cytochrome P450 monooxygenase; cytochrome P450, 20a1; cytochrome P450, family 20, subfamily A, polypeptide 1
Gene Aliases: A930011N14Rik; CYP-M; CYP20A1; Cypm; UNQ667/PRO1301
UniProt ID: (Human) Q6UW02, (Mouse) Q8BKE6, (Rat) Q6P7D4
Entrez Gene ID: (Human) 57404, (Mouse) 77951, (Rat) 316435
monooxygenase activity
iron ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
heme binding
oxidoreductase activity
fluorene oxygenase activity
mono-butyltin dioxygenase activity
tri-n-butyltin dioxygenase activity
di-n-butyltin dioxygenase activity
methylsilanetriol hydroxylase activity
methyl tertiary butyl ether 3-monooxygenase activity
4-nitrocatechol 4-monooxygenase activity
4-chlorophenoxyacetate monooxygenase activity
tert-butanol 2-monooxygenase activity
alpha-pinene monooxygenase activity
dimethylsilanediol hydroxylase activity
ammonia monooxygenase activity
hydroxymethylsilanetriol oxidase activity
2-hydroxyisobutyrate 3-monooxygenase activity
alpha-pinene dehydrogenase activity
bisphenol A hydroxylase B activity
2,2-bis(4-hydroxyphenyl)-1-propanol hydroxylase activity
9-fluorenone-3,4-dioxygenase activity
anthracene 9,10-dioxygenase activity
2-(methylthio)benzothiazole monooxygenase activity
2-hydroxybenzothiazole monooxygenase activity
benzothiazole monooxygenase activity
2,6-dihydroxybenzothiazole monooxygenase activity
pinacolone 5-monooxygenase activity
thioacetamide S-oxygenase activity
thioacetamide S-oxide S-oxygenase activity
endosulfan monooxygenase I activity
N-nitrodimethylamine hydroxylase activity
4-(1-ethyl-1,4-dimethyl-pentyl)phenol monoxygenase activity
endosulfan ether monooxygenase activity
pyrene 4,5-monooxygenase activity
pyrene 1,2-monooxygenase activity
1-hydroxypyrene 6,7-monooxygenase activity
1-hydroxypyrene 7,8-monooxygenase activity
phenylboronic acid monooxygenase activity
spheroidene monooxygenase activity
metal ion binding
oxidoreductase
oxygenase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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