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Invitrogen
CYP27A1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
CYP27A1 Antibody (PA5-27946) in IHC (P)
CYP27A1 Polyclonal Antibody detects CYP27A1 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CYP27A1 Polyclonal Antibody (Product # PA5-27946) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-27946
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 154 and 460 of Human CYP27A1
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545422
Product Specific Information
Recommended positive controls: A549, mouse liver, rat liver.
Predicted reactivity: Pig (83%), Rabbit (83%), Rhesus Monkey (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CYP27A1 participates in the degradation of cholesterol to bile acids in both the classic and acidic pathways. [1] It is the initiating enzyme in the acidic pathway to bile acids, yielding oxysterols by introducing a hydroxyl group to the carbon at the 27 position in cholesterol. In the acidic pathway, it produces 27-hydroxycholesterol from cholesterol whereas in the classic or neutral pathway, it produces 3beta-hydroxy-5-cholestenoic acid. It is also involved in the metabolism of vitamin D3. While CYP27A1 is present in many different tissues, its function in these tissues is largely uncharacterized. In macrophages, 27-hydroxycholesterol generated by this enzyme may be helpful against the production of inflammatory factors associated with cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; cholestanetriol 26-monooxygenase; cholesterol 27 hydroxylase; cyt; Cytochrome P-450C27/25; Cytochrome P450 27; cytochrome P450, 27; cytochrome P450, 27a1; cytochrome P450, family 27, subfamily A, polypeptide 1; cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1; Sterol 26-hydroxylase, mitochondrial; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase
Gene Aliases: 1300013A03Rik; CP27; CTX; CYP27; CYP27A1; P450C27
UniProt ID: (Human) Q02318, (Mouse) Q9DBG1, (Rat) P17178
Entrez Gene ID: (Human) 1593, (Mouse) 104086, (Rat) 301517
iron ion binding
steroid hydroxylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
heme binding
monooxygenase activity
oxidoreductase activity
vitamin D3 25-hydroxylase activity
cholesterol 26-hydroxylase activity
metal ion binding
cholestanetriol 26-monooxygenase activity
oxygenase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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