Immunofluorescent analysis of CYP4F11 showing staining in the endoplasmic reticulum of A549 cells. A549 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CYP4F11 polyclonal antibody (Product # PA5-30784) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 235 of Human CYP4F11|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30784 targets CYP4F11 in WB, IHC (P), and ICC applications and shows reactivity with Human samples.
The PA5-30784 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 235 of Human CYP4F11.
This gene, CYP4F11, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F2, is approximately 16 kb away. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
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