Invitrogen

Calcitonin Receptor Polyclonal Antibody

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Calcitonin Receptor Antibody in Immunocytochemistry (ICC/IF) — Fig. 1 Microglial cells express functional amylin receptors. a Primary cultures of human fetal microglia (HFMs) that are stained with microglial antibody, Iba-1 (green), and DyLight-594-labeled lectin (red). b These primary cultured HFMs were also stained for the two dimeric proteins that are components of the amylin receptor 3 (AMY3) subtype, the calcitonin receptor (CTR), and the receptor-associated membrane protein 3 (RAMP3). c Cultured HFMs were loaded with the fluorescent intracellular calcium dye, Fluo-8L-AM (green), and with lectin (red), an in vivo microglial marker. Arrowheads indicate cells from which Ca 2+ signals were recorded. The same cell culture is also stained with lectin (red). The field in c shows that a majority of cells are microglia. d Summary of data on intracellular calcium changes after human amylin (hAmylin) and Abeta 1-42 without and with application of the amylin receptor antagonist, AC253 in HFM (* p < 0.05, n = 123 cells in 12 culture wells from four different batch of the culture cells). The candidate traces for intracellular calcium changes are illustrated in e - i . Elevations of Ca 2+ induced by acute (30 s) application of either hAmylin (1 muM, e ) or Abeta 1-42 (1 muM, f ). The changes in Ca 2+ were blocked by AC253 (10 muM, g - i ). Traces correspond to cells identified in c with arrowheads. Scale bar = 20 mum

Calcitonin Receptor Antibody in Western Blot (WB) — Additional file 2: Figure S1. A, Western blot showing AMY3 transfected HEK293 cells demonstrate a marked increase in level of expression of CTR and RAMP3 proteins compared to wild-type (WT) HEK cells. B, in BV2 cells, RAMP3 protein expression shows a marked decreased after RAMP3 siRNA transfection compared to the control non-transfected cells. (JPEG 1495 kb)

Product Details

PA1-84457

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000-1:5,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:500	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
ELISA (ELISA)	1:100,000	-

Product Specifications
Species Reactivity	Fish, Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide derived from a C-terminal sequence of rat calcitonin receptor View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	<0.1% sodium azide
Storage conditions	4°C or -20°C if preferred
RRID	AB_2228378

Product Specific Information

The use of 4% paraformaldehyde fixation, rather than formalin fixation, is recommended. A sensitive detection system is also recommended, such as a catalyzed signal amplification system. A suggested positive control for immunohistochemical applications is kidney, cultured osteoclasts.

Rabbit anti Rat Calcitonin Receptor antibody recognizes the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems.

Target Information

The Calcitonin Receptor inhibits bone resorption. This receptor is expressed during development and is involved in morphogenesis. Three alternatively spliced Calcitonin Receptor isoforms have been identified. In addition to binding calcitonin, the Calcitonin Receptor can complex with RAMP3 (receptor activity modifying protein) to bind amylin, which regulates pancreatic islet function and is involved in Type II diabetes. The Calcitonin Receptor has been reported in artery, blood, bone, brain, kidney, lung, placenta, prostate, skeletal muscle, and thyroid. ESTs have been isolated from bone, embryo, kidney, and melanocyte/uterus/fetal heart libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.