Invitrogen

Calcitonin Receptor Polyclonal Antibody

Product Image Gallery

Calcitonin Receptor Antibody — Fig. 1 Microglial cells express functional amylin receptors. a Primary cultures of human fetal microglia (HFMs) that are stained with microglial antibody, Iba-1 (green), and DyLight-594-labeled lectin (red). b These primary cultured HFMs were also stained for the two dimeric proteins that are components of the amylin receptor 3 (AMY3) subtype, the calcitonin receptor (CTR), and the receptor-associated membrane protein 3 (RAMP3). c Cultured HFMs were loaded with the fluorescent intracellular calcium dye, Fluo-8L-AM (green), and with lectin (red), an in vivo microglial marker. Arrowheads indicate cells from which Ca 2+ signals were recorded. The same cell culture is also stained with lectin (red). The field in c shows that a majority of cells are microglia. d Summary of data on intracellular calcium changes after human amylin (hAmylin) and Abeta 1-42 without and with application of the amylin receptor antagonist, AC253 in HFM (* p < 0.05, n = 123 cells in 12 culture wells from four different batch of the culture cells). The candidate traces for intracellular calcium changes are illustrated in e - i . Elevations of Ca 2+ induced by acute (30 s) application of either hAmylin (1 muM, e ) or Abeta 1-42 (1 muM, f ). The changes in Ca 2+ were blocked by AC253 (10 muM, g - i ). Traces correspond to cells identified in c with arrowheads. Scale bar = 20 mum

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay Dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:50

Immunocytochemistry (ICC)

View 1 publication 1 publication

Immunofluorescence (IF)

Assay Dependent

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Rat, Fish, Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from a C-terminal sequence of rat calcitonin receptor

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

4°C or -20°C if preferred

RRID

AB_2228378

Product Specific Information

The use of 4% paraformaldehyde fixation, rather than formalin fixation, is recommended. A sensitive detection system is also recommended, such as a catalyzed signal amplification system. A suggested positive control for immunohistochemical applications is kidney, cultured osteoclasts.

Rabbit anti Rat Calcitonin Receptor antibody recognizes the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems.

Target Information

The Calcitonin Receptor inhibits bone resorption. This receptor is expressed during development and is involved in morphogenesis. Three alternatively spliced Calcitonin Receptor isoforms have been identified. In addition to binding calcitonin, the Calcitonin Receptor can complex with RAMP3 (receptor activity modifying protein) to bind amylin, which regulates pancreatic islet function and is involved in Type II diabetes. The Calcitonin Receptor has been reported in artery, blood, bone, brain, kidney, lung, placenta, prostate, skeletal muscle, and thyroid. ESTs have been isolated from bone, embryo, kidney, and melanocyte/uterus/fetal heart libraries.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.