Invitrogen

Calcium Sensing Receptor Polyclonal Antibody

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Calcium Sensing Receptor Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Calcium Sensing Receptor in 4% paraformaldehyde-fixed HeLa cells using Calcium Sensing Receptor polyclonal antibody (Product # PA5-85137) at a dilution of 1:200.

Calcium Sensing Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Calcium Sensing Receptor (Product # PA5-85137) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Calcium Sensing Receptor Antibody in Western Blot (WB) — Western Blot using Calcium Sensing Receptor Polyclonal Antibody (Product # PA5-85137). Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor Polyclonal Antibody (Product # PA5-85137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Product Details

PA5-85137

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human Calcium Sensing Receptor.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300, 0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792284

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (93%), Rat (94%), Dog (95%), Cat (95%), Pig (95%), Bovine (95%).

Positive Control: 293T, A431, H1299, HeLa, mouse brain, mouse placenta.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G- protein that activates a phosphatidylinositol-calcium second messenger system.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ca sensing receptor; calcium-sensing receptor; CaR; CaSR; CaSR antibody; cation sensing receptor; Extracellular calcium-sensing receptor; G protein coupled receptor, family C, group 2, member A; G protein-coupled receptor, family C, group 2, member A; parathyroid Ca(2+)-sensing receptor 1; Parathyroid cell calcium-sensing receptor; Parathyroid cell calcium-sensing receptor 1; PCaR1

Gene Aliases: CAR; CASR; EIG8; FHH; FIH; GPRC2A; HHC; HHC1; HYPOC1; NSHPT; PCAR1

UniProt ID: (Human) P41180, (Mouse) Q9QY96

Entrez Gene ID: (Human) 846, (Mouse) 12374

Molecular Function: G-protein coupled receptor