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Immunofluorescent analysis of Calreticulin showing staining in the endoplasmic reticulum of Jurkat cells. Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Calreticulin polyclonal antibody (Product # PA5-34786) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 214 of Calreticulin (Uniprot ID#P27797)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34786 targets Calreticulin in IHC (P) and WB applications and shows reactivity with Human and Mouse samples.
The PA5-34786 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 214 of Calreticulin (Uniprot ID#P27797).
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
autoantigen Ro calreticulin; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA
CALR; Calregulin; cC1qR; CRT; CRTC; HEL-S-99n; RO; SSA