PA1-743 detects cannabinoid receptor 1 (CB1) from human and rat tissues as well as transfected rat CB1.
PA1-743 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~52 kDa protein representing CB1 from rat brain homogenate. Immunocytochemical staining of CB1 in AtT20 cells transfected with the rat CB1 gene with PA1-743 yields a pattern consistent with plasma membrane staining.
PA1-743 antigen is a fusion protein containing the first 99 amino acid residues of human CB1.
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P/Q type calcium channels.
CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Brain-type cannabinoid receptor; CANN6; Cannabinoid receptor 1; cannabinoid receptor 1 (brain); CB-R; CB1; central cannabinoid receptor; Cnr1
Gene Aliases: CANN6; CB-R; CB1; CB1A; CB1K5; CB1R; CNR; CNR1; Skr6; SKR6R