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Cardiac Troponin I Polyclonal Antibody

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Antibody Testing Data (9)

Cardiac Troponin I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

Cardiac Troponin I Antibody (21652-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue slide using (Product # 21652-1-AP) Cardiac Troponin I antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cardiac Troponin I Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Cardiac Troponin I Antibody in Western Blot (WB)

Cardiac Troponin I Antibody in Immunoprecipitation (IP)

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Product Details

21652-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 14 publications 14 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cardiac Troponin I Fusion Protein Ag16365 (1-110 aa encoded by BC096165)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MADGSSDAA REPRPAPAPI RRRSSNYRAY ATEPHAKKKS KISASRKLQL KTLLLQIAKQ ELEREAEERR GEKGRALSTR CQPLELAGLG FAELQDLCRQ LHARVDKVDE E (1-110 aa encoded by BC096165)

Target Information

Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Platelet Internalization Mediates Ferroptosis in Myocardial Infarction.

Arteriosclerosis, thrombosis, and vascular biology

Miao S,Zhang Q,Ding W,Hou B,Su Z,Li M,Yang L,Zhang J,Chang W,Wang J

Wed Feb 01 00:00:00 EST 2023

Pathological implication of CaMKII in NF-κB pathway and SASP during cardiomyocytes senescence.

Mechanisms of ageing and development

Li T,Meng Y,Ding P,Wang H,Liu J,Xia C,Chen Y,Li J

Sun Jan 01 00:00:00 EST 2023

G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region.

Molecular medicine reports

Xu P,Jin K,Zhou J,Gu J,Gu X,Dong L,Sun X

Wed Feb 01 00:00:00 EST 2023

G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region.

Molecular medicine reports

Xu P,Jin K,Zhou J,Gu J,Gu X,Dong L,Sun X

Wed Feb 01 00:00:00 EST 2023

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

Aging

Li C,Zheng Y,Liu Y,Jin GH,Pan H,Yin F,Wu J

Thu Jan 20 00:00:00 EST 2022

Cannabidiol attenuates methamphetamine-induced cardiac inflammatory response through the PKA/CREB pathway in rats.

Annals of translational medicine

Nie Q,Dong W,Shen B,Yang G,Yu H,Zhang R,Peng Y,Yu Y,Hong S,Li L

Thu Sep 01 00:00:00 EDT 2022

Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF-κB signaling pathways.

JCI insight

Yang J,Li H,Zhang C,Zhou Y

Tue Feb 08 00:00:00 EST 2022

Pharmacological activation of estrogenic receptor G protein-coupled receptor 30 attenuates angiotensin II-induced atrial fibrosis in ovariectomized mice by modulating TGF-β1/smad pathway.

Molecular biology reports

Liu D,Zhan Y,Ono K,Yin Y,Wang L,Wei M,Ji L,Liu M,Liu G,Zhou X,Zheng M

Fri Jul 01 00:00:00 EDT 2022

Pharmacological activation of estrogenic receptor G protein-coupled receptor 30 attenuates angiotensin II-induced atrial fibrosis in ovariectomized mice by modulating TGF-β1/smad pathway.

Molecular biology reports

Liu D,Zhan Y,Ono K,Yin Y,Wang L,Wei M,Ji L,Liu M,Liu G,Zhou X,Zheng M

Fri Jul 01 00:00:00 EDT 2022

Engineering of thick human functional myocardium via static stretching and electrical stimulation.

iScience

Pretorius D,Kahn-Krell AM,LaBarge WC,Lou X,Zhang J

Fri Mar 18 00:00:00 EDT 2022

Triggering receptor expressed on myeloid cells-2 promotes survival of cardiomyocytes after myocardial ischemic injury through PI3K/AKT pathway.

Cardiovascular diagnosis and therapy

Fu C,Xu Q,Liu J,Tang S,Liu C,Cao Y

Tue Feb 01 00:00:00 EST 2022

Epididymal white adipose tissue promotes angiotensin II-induced cardiac fibrosis in an exosome-dependent manner.

Translational research : the journal of laboratory and clinical medicine

Su M,Li W,Yuan Y,Liu S,Liang C,Liu HE,Zhang R,Liu Y,Sun LI,Wei Y,Li C,Han X,Hao H,Zhao X,Luo Y,Yan S,Pan Z,Li Y

Sat Oct 01 00:00:00 EDT 2022

Hydroxyurea protects against diabetic cardiomyopathy by inhibiting inflammation and apoptosis.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhou Y,Lu Q

Thu Sep 01 00:00:00 EDT 2022

Exploration of the amino acid metabolic signature in anthracycline-induced cardiotoxicity using an optimized targeted metabolomics approach based on UPLC-MS/MS.

Naunyn-Schmiedeberg's archives of pharmacology

Li W,Li S,Cao Z,Sun Y,Qiu W,Jia M,Su M

Sat Oct 01 00:00:00 EDT 2022

A Neonatal Mouse Model for Pressure Overload: Myocardial Response Corresponds to Severity.

Frontiers in cardiovascular medicine

Gu J,Chen X,Jin Y,Liu M,Xu Q,Liu X,Luo Z,Ling S,Liu N,Liu S

Tue Jun 08 00:00:00 EDT 2021

A Modified Surgical Ventricular Reconstruction in Post-infarction Mice Persistently Alleviates Heart Failure and Improves Cardiac Regeneration.

Frontiers in cardiovascular medicine

Ma S,Yan J,Yang D,Liao W,Bin J,Lin H,Liao Y

Tue Jan 11 00:00:00 EST 2022

Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model.

Viruses

Ji W,Zhu P,Liang R,Zhang L,Zhang Y,Wang Y,Zhang W,Tao L,Chen S,Yang H,Jin Y,Duan G

Wed Aug 11 00:00:00 EDT 2021

Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model.

Viruses

Ji W,Zhu P,Liang R,Zhang L,Zhang Y,Wang Y,Zhang W,Tao L,Chen S,Yang H,Jin Y,Duan G

Wed Aug 11 00:00:00 EDT 2021

Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.

EBioMedicine

Li C,Liu F,Liu S,Pan H,Du H,Huang J,Xie Y,Li Y,Zhao R,Wei Y

Sun Mar 01 00:00:00 EST 2020

CD38 Causes Autophagic Flux Inhibition and Cardiac Dysfunction Through a Transcriptional Inhibition Pathway Under Hypoxia/Ischemia Conditions.

Frontiers in cell and developmental biology

Zhang X,Li L,Zhang Q,Wei Q,Lin J,Jia J,Zhang J,Yan T,Lv Y,Jiang X,Zhang P,Song H,Zhang D,Huang Y

Mon Sep 28 00:00:00 EDT 2020

Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use.

Biosensors & bioelectronics

Chen LC,Wang E,Tai CS,Chiu YC,Li CW,Lin YR,Lee TH,Huang CW,Chen JC,Chen WL

Fri May 01 00:00:00 EDT 2020

Localized injection of miRNA-21-enriched extracellular vesicles effectively restores cardiac function after myocardial infarction.

Theranostics

Song Y,Zhang C,Zhang J,Jiao Z,Dong N,Wang G,Wang Z,Wang L

Thu Jun 25 00:00:00 EDT 2020

Exosomes derived from cardiac progenitor cells attenuate CVB3-induced apoptosis via abrogating the proliferation of CVB3 and modulating the mTOR signaling pathways.

Cell death & disease

Li X,Yang Z,Nie W,Jiang J,Li S,Li Z,Tian L,Ma X

Wed Sep 18 00:00:00 EDT 2019

Microtubule associated protein 4 phosphorylation leads to pathological cardiac remodeling in mice.

EBioMedicine

Li L,Zhang Q,Zhang X,Zhang J,Wang X,Ren J,Jia J,Zhang D,Jiang X,Zhang J,Mei H,Chen B,Hu J,Huang Y

Thu Nov 01 00:00:00 EDT 2018

Microtubule associated protein 4 phosphorylation leads to pathological cardiac remodeling in mice.

EBioMedicine

Li L,Zhang Q,Zhang X,Zhang J,Wang X,Ren J,Jia J,Zhang D,Jiang X,Zhang J,Mei H,Chen B,Hu J,Huang Y

Thu Nov 01 00:00:00 EDT 2018

PGC-1α Participates in the Protective Effect of Chronic Intermittent Hypobaric Hypoxia on Cardiomyocytes.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Gu S,Hua H,Guo X,Jia Z,Zhang Y,Maslov LN,Zhang X,Ma H

Mon Nov 26 00:00:00 EST 2018

PGC-1α Participates in the Protective Effect of Chronic Intermittent Hypobaric Hypoxia on Cardiomyocytes.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Gu S,Hua H,Guo X,Jia Z,Zhang Y,Maslov LN,Zhang X,Ma H

Mon Nov 26 00:00:00 EST 2018

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Theranostics

Shi J,Bei Y,Kong X,Liu X,Lei Z,Xu T,Wang H,Xuan Q,Chen P,Xu J,Che L,Liu H,Zhong J,Sluijter JP,Li X,Rosenzweig A,Xiao J

Mon Oct 23 00:00:00 EDT 2017

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Theranostics

Shi J,Bei Y,Kong X,Liu X,Lei Z,Xu T,Wang H,Xuan Q,Chen P,Xu J,Che L,Liu H,Zhong J,Sluijter JP,Li X,Rosenzweig A,Xiao J

Mon Oct 23 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); MGC116817; Tpn1; troponin 1, type 3; troponin I type 3 (cardiac); Troponin I, cardiac muscle

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Gene Aliases: CMD1FF; CMD2A; CMH7; Ctni; RCM1; Tn1; TnI; TNNC1; TNNI3

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UniProt ID: (Human) P19429, (Rat) P23693, (Mouse) P48787

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Entrez Gene ID: (Human) 7137, (Rat) 29248, (Mouse) 21954

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Function(s)

actin binding protein binding calcium channel inhibitor activity protein kinase binding protein domain specific binding troponin C binding troponin T binding metal ion binding calcium-dependent protein binding non-motor actin binding protein

Process(es)

vasculogenesis regulation of systemic arterial blood pressure by ischemic conditions skeletal muscle contraction cellular calcium ion homeostasis regulation of smooth muscle contraction heart development muscle filament sliding negative regulation of ATPase activity ventricular cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction regulation of cardiac conduction regulation of muscle contraction striated muscle contraction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cardiac Troponin I Polyclonal Antibody

Cardiac Troponin I Antibody (21652-1-AP) in IHC (P)

Product Details

21652-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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