Immunofluorescent analysis of Caspase 8 (green) HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Caspase 8 polyclonal antibody (Product # PA1-29159) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat-anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope corresponding to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29159 detects Caspase 8 from human, mouse, rat samples.
PA1-29159 has been successfully used in IF/ICC, immunohistochemistry (paraffin tissue), immunoprecipitation, and Western blot applications.
The PA1-29159 immunogen corresponds to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin.
Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the proform of the Caspase 8 protein. Recruitment of Caspase 8 to the Fas receptor results in oligomerization of the Caspase 8 protein, which in turn drives up its autoactivation through "self-cleavage". Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby commiting the cell to undergo apoptosis. Reacts with Jurkat cells and Tonsil.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.