Immunofluorescence analysis of Cdc14A was performed using 70% confluent log phase HCT 116 cells treated with MG-132 (10 micromolar for 16h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cdc14A Rabbit Polyclonal Antibody (Product # 34-8100) at 2µg/mlin 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the untreated cells with no expression of Cdc14A. Panel f shows the primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the human Cdc14A protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion.
34-8100 was used in immunocytochemistry and western blot to study regulation of adhesion and cell migration by recruitment to the leading edge by human phosphatase CDC14A
|Chen NP,Uddin B,Voit R,Schiebel E||Proceedings of the National Academy of Sciences of the United States of America (113:990)||2016|
|Human||Not Cited||The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.||Cho HP,Liu Y,Gomez M,Dunlap J,Tyers M,Wang Y||Molecular and cellular biology (25:4541)||2005|
The CDC14A phosphatase regulates oocyte maturation in mouse.
34-8100 was used in immunocytochemistry to test if CDC14A is required for meiosis in female mice
|Schindler K,Schultz RM||Cell cycle (Georgetown, Tex.) (8:1090)||2009|
||The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.||Cho HP,Liu Y,Gomez M,Dunlap J,Tyers M,Wang Y||Molecular and cellular biology (25:4541)||2005|