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|Tested species reactivity||Chicken|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 15 publications below|
These goat anti-chicken IgY (H+L) whole secondary antibodies have been affinity-purified and show minimum cross-reactivity. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 594 dye is a bright, red-fluorescent dye with excitation ideally suited to the 594 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 594 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.
Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.
Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Rbm20 regulates titin alternative splicing as a splicing repressor.||Li S,Guo W,Dewey CN,Greaser ML||Nucleic acids research (41:2659)||2013|
|Not Applicable||Not Cited||Actin polymerization controls the activation of multidrug efflux at fertilization by translocation and fine-scale positioning of ABCB1 on microvilli.||Whalen K,Reitzel AM,Hamdoun A||Molecular biology of the cell (23:3663)||2012|
|Not Applicable||Not Cited||Vagal afferent stimulation activates astrocytes in the nucleus of the solitary tract via AMPA receptors: evidence of an atypical neural-glial interaction in the brainstem.||McDougal DH,Hermann GE,Rogers RC||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:14037)||2011|
|Not Applicable||Not Cited||Generation of keratinocytes from normal and recessive dystrophic epidermolysis bullosa-induced pluripotent stem cells.||Itoh M,Kiuru M,Cairo MS,Christiano AM||Proceedings of the National Academy of Sciences of the United States of America (108:8797)||2011|
|Not Applicable||Not Cited||Dot1a contains three nuclear localization signals and regulates the epithelial Na+ channel (ENaC) at multiple levels.||Reisenauer MR,Wang SW,Xia Y,Zhang W||American journal of physiology. Renal physiology (299:F63)||2010|
|Not Applicable||Not Cited||Analysis of a charge cluster mutation of herpes simplex virus type 1 UL34 and its extragenic suppressor suggests a novel interaction between pUL34 and pUL31 that is necessary for membrane curvature around capsids.||Roller RJ,Bjerke SL,Haugo AC,Hanson S||Journal of virology (84:3921)||2010|
|Not Applicable||Not Cited||Growth regulation by Dpp: an essential role for Brinker and a non-essential role for graded signaling levels.||Schwank G,Restrepo S,Basler K||Development (Cambridge, England) (135:4003)||2008|
|Not Applicable||Not Cited||Emerin is hyperphosphorylated and redistributed in herpes simplex virus type 1-infected cells in a manner dependent on both UL34 and US3.||Leach N,Bjerke SL,Christensen DK,Bouchard JM,Mou F,Park R,Baines J,Haraguchi T,Roller RJ||Journal of virology (81:10792)||2007|
|Not Applicable||Not Cited||Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry.||Ahmed Z,Shaw G,Sharma VP,Yang C,McGowan E,Dickson DW||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (55:687)||2007|
|Not Applicable||Not Cited||Specific role of the truncated betaIV-spectrin Sigma6 in sodium channel clustering at axon initial segments and nodes of ranvier.||Uemoto Y,Suzuki S,Terada N,Ohno N,Ohno S,Yamanaka S,Komada M||The Journal of biological chemistry (282:6548)||2007|
|Not Applicable||Not Cited||Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line.||Mikhaylova LM,Boutanaev AM,Nurminsky DI||Proceedings of the National Academy of Sciences of the United States of America (103:11975)||2006|
|Not Applicable||Not Cited||Association of excitatory amino acid transporters, especially EAAT2, with cholesterol-rich lipid raft microdomains: importance for excitatory amino acid transporter localization and function.||Butchbach ME,Tian G,Guo H,Lin CL||The Journal of biological chemistry (279:34388)||2004|
|Not Applicable||Not Cited||Poly(beta-amino ester)s promote cellular uptake of heparin and cancer cell death.||Berry D,Lynn DM,Sasisekharan R,Langer R||Chemistry & biology (11:487)||2004|
|Not Applicable||Not Cited||Ultrathin cryosections: an important tool for immunofluorescence and correlative microscopy.||Takizawa T,Robinson JM||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (51:707)||2003|
|Not Applicable||Not Cited||Activation and transcriptional interaction between agr RNAII and RNAIII in Staphylococcus aureus in vitro and in an experimental endocarditis model.||Xiong YQ,Van Wamel W,Nast CC,Yeaman MR,Cheung AL,Bayer AS||The Journal of infectious diseases (186:668)||2002|