Proteintech

Choline kinase alpha Polyclonal Antibody

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Antibody Testing Data (7)

Choline kinase alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Choline kinase alpha Antibody (13520-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 13520-1-AP) (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Choline kinase alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Choline kinase alpha Antibody in Western Blot (WB)

Choline kinase alpha Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13520-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Choline kinase alpha Fusion Protein Ag4761 (139-439 aa encoded by BC036471)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EPRKVLLRL YGAILQVGAE AMVLESVMFA ILAERSLGPK LYGIFPQGRL EQFIPSRRLD TEELGLPDIS AEIAEKMATF HGMKMPFNKE PKWLFGTMEK YLKEVLRIKF TEESRIKKLH KLLSYNLPLE LENLRSLLES TPSPVVFCHN DCQEGNILLL EGRENSEKQK LMLIDFEYSS YNYRGFDIGN HFCEWMYDYS YEKYPFFRAN IRKYPTKKQQ LHFISSYLPA FQNDFENLST EEKSIIKEEM LLEVNRFALA SHFLWGQWSI VQAKISSIEF GYMDYAQARF DAYFHQKRKL GV (139-439 aa encoded by BC036471)

Target Information

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase alpha is the initial enzyme in the sequence and may play a regulatory role. This protein also catalyzes the phosphorylation of ethanolamine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel function of Roxadustat (FG-4592) as an anti-shock drug in sepsis by regulating mitochondrial oxidative stress and energy metabolism.

Biochimica et biophysica acta. General subjects

Long G,Pei Z,Wu M,Wei K,Du Y,Wang Q,Zhang Y,Huang S,Chen H,Xia W,Jia Z

Sun Jan 01 00:00:00 EST 2023

Novel function of Roxadustat (FG-4592) as an anti-shock drug in sepsis by regulating mitochondrial oxidative stress and energy metabolism.

Biochimica et biophysica acta. General subjects

Long G,Pei Z,Wu M,Wei K,Du Y,Wang Q,Zhang Y,Huang S,Chen H,Xia W,Jia Z

Sun Jan 01 00:00:00 EST 2023

Novel function of Roxadustat (FG-4592) as an anti-shock drug in sepsis by regulating mitochondrial oxidative stress and energy metabolism.

Biochimica et biophysica acta. General subjects

Long G,Pei Z,Wu M,Wei K,Du Y,Wang Q,Zhang Y,Huang S,Chen H,Xia W,Jia Z

Sun Jan 01 00:00:00 EST 2023

Novel function of Roxadustat (FG-4592) as an anti-shock drug in sepsis by regulating mitochondrial oxidative stress and energy metabolism.

Biochimica et biophysica acta. General subjects

Long G,Pei Z,Wu M,Wei K,Du Y,Wang Q,Zhang Y,Huang S,Chen H,Xia W,Jia Z

Sun Jan 01 00:00:00 EST 2023

Altered choline level in atherosclerotic lesions: Upregulation of choline transporter-like protein 1 in human coronary unstable plaque.

PloS one

Nakamura E,Maekawa K,Saito Y,Matsumoto T,Ogawa M,Komohara Y,Asada Y,Yamashita A

Wed Feb 22 00:00:00 EST 2023

Twist alters the breast tumor microenvironment via choline kinase to facilitate an aggressive phenotype.

Molecular and cellular biochemistry

Vesuna F,Penet MF,Mori N,Bhujwalla ZM,Raman V

Sat Apr 01 00:00:00 EDT 2023

Functional roles of FAP-α in metabolism, migration and invasion of human cancer cells.

Frontiers in oncology

Mori N,Jin J,Krishnamachary B,Mironchik Y,Wildes F,Vesuna F,Barnett JD,Bhujwalla ZM

Sat Jul 01 00:00:00 EDT 2023

The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation.

Proceedings of the National Academy of Sciences of the United States of America

Ma Q,Jiang H,Ma L,Zhao G,Xu Q,Guo D,He N,Liu H,Meng Z,Liu J,Zhu L,Lin Q,Wu X,Li M,Luo S,Fang J,Lu Z

Tue Apr 11 00:00:00 EDT 2023

The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation.

Proceedings of the National Academy of Sciences of the United States of America

Ma Q,Jiang H,Ma L,Zhao G,Xu Q,Guo D,He N,Liu H,Meng Z,Liu J,Zhu L,Lin Q,Wu X,Li M,Luo S,Fang J,Lu Z

Tue Apr 11 00:00:00 EDT 2023

1,25(OH)₂D₃ alleviates LPS-induced preeclampsia-like rats impairment in the protective effect by TLR4/NF-kB pathway.

Placenta

Ma Y,Yang Y,Lv M,Zhang Y,He Q,Zhang Y,Su H,Deng X,Qian Y

Thu Dec 01 00:00:00 EST 2022

Crosstalk of FGFR1 signaling and choline metabolism promotes cell proliferation and survival in prostate cancer cells.

International journal of cancer

Fan Z,Ma J,Pan X,Zhao L,Wu Y,Lin H,Zhao Y,Jiang H,Pan T,Li X,Wang F,Wang C

Sun May 01 00:00:00 EDT 2022

Crosstalk of FGFR1 signaling and choline metabolism promotes cell proliferation and survival in prostate cancer cells.

International journal of cancer

Fan Z,Ma J,Pan X,Zhao L,Wu Y,Lin H,Zhao Y,Jiang H,Pan T,Li X,Wang F,Wang C

Sun May 01 00:00:00 EDT 2022

DHRS2 inhibits cell growth and metastasis in ovarian cancer by downregulation of CHKα to disrupt choline metabolism.

Cell death & disease

Li Z,Tan Y,Li X,Quan J,Bode AM,Cao Y,Luo X

Mon Oct 03 00:00:00 EDT 2022

DHRS2 inhibits cell growth and metastasis in ovarian cancer by downregulation of CHKα to disrupt choline metabolism.

Cell death & disease

Li Z,Tan Y,Li X,Quan J,Bode AM,Cao Y,Luo X

Mon Oct 03 00:00:00 EDT 2022

DHRS2 inhibits cell growth and metastasis in ovarian cancer by downregulation of CHKα to disrupt choline metabolism.

Cell death & disease

Li Z,Tan Y,Li X,Quan J,Bode AM,Cao Y,Luo X

Mon Oct 03 00:00:00 EDT 2022

DHRS2 inhibits cell growth and metastasis in ovarian cancer by downregulation of CHKα to disrupt choline metabolism.

Cell death & disease

Li Z,Tan Y,Li X,Quan J,Bode AM,Cao Y,Luo X

Mon Oct 03 00:00:00 EDT 2022

Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.

Frontiers in cell and developmental biology

Kong M,Dong W,Xu H,Fan Z,Miao X,Guo Y,Li C,Ye Q,Wang Y,Xu Y

Tue Aug 24 00:00:00 EDT 2021

Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.

Cell metabolism

Sanchez-Lopez E,Zhong Z,Stubelius A,Sweeney SR,Booshehri LM,Antonucci L,Liu-Bryan R,Lodi A,Terkeltaub R,Lacal JC,Murphy AN,Hoffman HM,Tiziani S,Guma M,Karin M

Tue Jun 04 00:00:00 EDT 2019

Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.

Cell metabolism

Sanchez-Lopez E,Zhong Z,Stubelius A,Sweeney SR,Booshehri LM,Antonucci L,Liu-Bryan R,Lodi A,Terkeltaub R,Lacal JC,Murphy AN,Hoffman HM,Tiziani S,Guma M,Karin M

Tue Jun 04 00:00:00 EDT 2019

Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis.

Nature communications

Sołtysik K,Ohsaki Y,Tatematsu T,Cheng J,Fujimoto T

Mon Jan 28 00:00:00 EST 2019

Preclinical Development of a Nontoxic Oral Formulation of Monoethanolamine, a Lipid Precursor, for Prostate Cancer Treatment.

Clinical cancer research : an official journal of the American Association for Cancer Research

Saxena R,Yang C,Rao M,Turaga RC,Garlapati C,Gundala SR,Myers K,Ghareeb A,Bhattarai S,Kamalinia G,Bristi S,Su D,Gadda G,Rida PCG,Cantuaria GH,Aneja R

Sat Jul 15 00:00:00 EDT 2017

Preclinical Development of a Nontoxic Oral Formulation of Monoethanolamine, a Lipid Precursor, for Prostate Cancer Treatment.

Clinical cancer research : an official journal of the American Association for Cancer Research

Saxena R,Yang C,Rao M,Turaga RC,Garlapati C,Gundala SR,Myers K,Ghareeb A,Bhattarai S,Kamalinia G,Bristi S,Su D,Gadda G,Rida PCG,Cantuaria GH,Aneja R

Sat Jul 15 00:00:00 EDT 2017

Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice.

Lipids in health and disease

Ming YN,Zhang JY,Wang XL,Li CM,Ma SC,Wang ZY,Liu XL,Li XB,Mao YM

Mon Aug 14 00:00:00 EDT 2017

Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice.

Lipids in health and disease

Ming YN,Zhang JY,Wang XL,Li CM,Ma SC,Wang ZY,Liu XL,Li XB,Mao YM

Mon Aug 14 00:00:00 EDT 2017

Investigation of discriminant metabolites in tamoxifen-resistant and choline kinase-alpha-downregulated breast cancer cells using 1H-nuclear magnetic resonance spectroscopy.

PloS one

Kim HS,Tian L,Kim H,Moon WK

Mon Oct 02 00:00:00 EDT 2017

Investigation of discriminant metabolites in tamoxifen-resistant and choline kinase-alpha-downregulated breast cancer cells using 1H-nuclear magnetic resonance spectroscopy.

PloS one

Kim HS,Tian L,Kim H,Moon WK

Mon Oct 02 00:00:00 EDT 2017

The Tumor Microenvironment Modulates Choline and Lipid Metabolism.

Frontiers in oncology

Mori N,Wildes F,Takagi T,Glunde K,Bhujwalla ZM

Fri Mar 11 00:00:00 EST 2022

The Tumor Microenvironment Modulates Choline and Lipid Metabolism.

Frontiers in oncology

Mori N,Wildes F,Takagi T,Glunde K,Bhujwalla ZM

Fri Mar 11 00:00:00 EST 2022

Choline kinase inhibition in rheumatoid arthritis.

Annals of the rheumatic diseases

Guma M,Sanchez-Lopez E,Lodi A,Garcia-Carbonell R,Tiziani S,Karin M,Lacal JC,Firestein GS

Wed Jul 01 00:00:00 EDT 2015

Choline kinase inhibition in rheumatoid arthritis.

Annals of the rheumatic diseases

Guma M,Sanchez-Lopez E,Lodi A,Garcia-Carbonell R,Tiziani S,Karin M,Lacal JC,Firestein GS

Wed Jul 01 00:00:00 EDT 2015

Choline kinase inhibition in rheumatoid arthritis.

Annals of the rheumatic diseases

Guma M,Sanchez-Lopez E,Lodi A,Garcia-Carbonell R,Tiziani S,Karin M,Lacal JC,Firestein GS

Wed Jul 01 00:00:00 EDT 2015

Choline kinase inhibition in rheumatoid arthritis.

Annals of the rheumatic diseases

Guma M,Sanchez-Lopez E,Lodi A,Garcia-Carbonell R,Tiziani S,Karin M,Lacal JC,Firestein GS

Wed Jul 01 00:00:00 EDT 2015

Choline kinase-α protein and phosphatidylcholine but not phosphocholine are required for breast cancer cell survival.

NMR in biomedicine

Mori N,Wildes F,Kakkad S,Jacob D,Solaiyappan M,Glunde K,Bhujwalla ZM

Tue Dec 01 00:00:00 EST 2015

Choline kinase-α protein and phosphatidylcholine but not phosphocholine are required for breast cancer cell survival.

NMR in biomedicine

Mori N,Wildes F,Kakkad S,Jacob D,Solaiyappan M,Glunde K,Bhujwalla ZM

Tue Dec 01 00:00:00 EST 2015

Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.

Molecular biology of the cell

Aitchison AJ,Arsenault DJ,Ridgway ND

Sat Aug 15 00:00:00 EDT 2015

Downregulation of Choline Kinase-Alpha Enhances Autophagy in Tamoxifen-Resistant Breast Cancer Cells.

PloS one

Kim HS,Tian L,Jung M,Choi SK,Sun Y,Kim H,Moon WK

Fri Jun 10 00:00:00 EDT 2016

Downregulation of Choline Kinase-Alpha Enhances Autophagy in Tamoxifen-Resistant Breast Cancer Cells.

PloS one

Kim HS,Tian L,Jung M,Choi SK,Sun Y,Kim H,Moon WK

Fri Jun 10 00:00:00 EDT 2016

ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.

The Journal of biological chemistry

Arsenault DJ,Yoo BH,Rosen KV,Ridgway ND

Fri Jan 04 00:00:00 EST 2013

ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.

The Journal of biological chemistry

Arsenault DJ,Yoo BH,Rosen KV,Ridgway ND

Fri Jan 04 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CHETK-alpha; Choline kinase alpha; choline kinase R; choline/ethanolamine kinase alpha; CK; EK; Ethanolamine kinase

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Gene Aliases: Chetk-alpha; CHK; CHKA; ChoK; CK; CK-R; CK/EK-alpha; CKI; Ckr; EK; EtnK-alpha

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UniProt ID: (Human) P35790, (Mouse) O54804, (Rat) Q01134

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Entrez Gene ID: (Human) 1119, (Mouse) 12660, (Rat) 29194

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Function(s)

choline kinase activity cholinesterase activity ethanolamine kinase activity signal transducer activity ATP binding drug binding choline binding protein homodimerization activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups kinase

Process(es)

ethanolamine metabolic process lipid metabolic process phosphatidylethanolamine biosynthetic process phosphatidylcholine biosynthetic process CDP-choline pathway lipid transport signal transduction response to toxic substance phosphorylation choline metabolic process response to 3-methylcholanthrene phospholipid biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Choline kinase alpha Polyclonal Antibody

Choline kinase alpha Antibody (13520-1-AP) in ICC/IF

Product Details

13520-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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