Invitrogen

Citrate Synthase Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (20) Citrate Synthase antibodies

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Datasheet

Protocols

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Citrate Synthase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Citrate synthase was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Citrate Synthetase Polyclonal Antibody (Product # PA5-22126) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Mitochondria (Panel c: red) was stained with Mitotracker Red CMXRos (Product # M7512). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Citrate Synthase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of Citrate Synthase was performed in paraffin-embedded mouse brain tissue using Citrate Synthase Polyclonal Antibody (Product # PA5-22126) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Citrate Synthase Antibody in Western Blot (WB) — Knockdown of Citrate Synthetase was achieved by transfecting Hep G2 with Citrate Synthetase specific siRNAs (Silencer® select Product # s3582). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Citrate Synthetase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Citrate Synthetase Polyclonal Antibody (Product # PA5-22126, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Citrate Synthetase.

Citrate Synthase Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of Citrate synthase protein from 293T whole cell extracts using 5 µg of Citrate synthase antibody (Product # PA5-22126).

Citrate Synthase Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with Citrate Synthetase siRNA and reduction of signal was observed in Western Blot using Citrate Synthetase Polyclonal Antibody (Product # PA5-22126). {KD}

Product Details

PA5-22126

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Chicken, Human, Mouse, Non-human primate, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 110 and 412 of Citrate synthetase (Uniprot ID#O75390) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.65 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11154268

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain, Rat muscle.

Predicted reactivity: Mouse (94%), Rat (93%), Zebrafish (90%), Xenopus laevis (91%), Pig (95%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metabolism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

mRNA Therapy Improves Metabolic and Behavioral Abnormalities in a Murine Model of Citrin Deficiency.

Molecular therapy : the journal of the American Society of Gene Therapy

Cao J,An D,Galduroz M,Zhuo J,Liang S,Eybye M,Frassetto A,Kuroda E,Funahashi A,Santana J,Mihai C,Benenato KE,Kumarasinghe ES,Sabnis S,Salerno T,Coughlan K,Miracco EJ,Levy B,Besin G,Schultz J,Lukacs C,Guey L,Finn P,Furukawa T,Giangrande PH,Saheki T,Martini PGV

PA5-22126 was used in Western Blotting to investigate the efficiacy of hCitrin-mRNA0LNP therapy in human cultured cells and in murine models of citrin deficiency.