Invitrogen

Citrate Synthase Polyclonal Antibody

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This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (11) Citrate Synthase antibodies

Datasheet

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Datasheet

Questions & Answers

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Citrate Synthase Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Citrate synthase was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Citrate Synthetase Polyclonal Antibody (Product # PA5-22126) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Mitochondria (Panel c: red) was stained with Mitotracker Red CMXRos (Product # M7512). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Citrate Synthase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Citrate synthase in the mitochondria of paraffin-embedded mouse intestine tissue using a Citrate synthase polyclonal antibody (Product # PA5-22126) at a dilution of 1:500.

Citrate Synthase Antibody in Western Blot (WB) — Knockdown of Citrate Synthetase was achieved by transfecting Hep G2 with Citrate Synthetase specific siRNAs (Silencer® select Product # s3582). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Citrate Synthetase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Citrate Synthetase Polyclonal Antibody (Product # PA5-22126, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Citrate Synthetase.

Citrate Synthase Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of Citrate synthase protein from 293T whole cell extracts using 5 µg of Citrate synthase antibody (Product # PA5-22126).

Citrate Synthase Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with Citrate Synthetase siRNA and reduction of signal was observed in Western Blot using Citrate Synthetase Polyclonal Antibody (Product # PA5-22126). {KD}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay-Dependent

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 110 and 412 of Citrate synthetase (Uniprot ID#O75390)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.84 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154268

Product Specific Information

PA5-22126 targets Citrate synthetase in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.

The PA5-22126 immunogen is a recombinant fragment corresponding to a region within amino acids 110 and 412 of Citrate synthase (Uniprot ID#O75390).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.