Immunohistochemistry analysis of Claudin-14 showing staining in the membrane and cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Claudin-14 polyclonal antibody (364200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the mid region of the human Claudin-14 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. Several transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||1:20||Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies.||Collins JE,Kirk A,Campbell SK,Mason J,Wilson SJ||Methods in molecular biology (Clifton, N.J.) (762:371)||2011|
|Human||Not Cited||Differential expression of claudin tight junction proteins in the human cortical nephron.||Kirk A,Campbell S,Bass P,Mason J,Collins J||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (25:2107)||2010|
Expression and function of tight junctions in the crypt epithelium of human palatine tonsils.
36-4200 was used in immunohistochemistry - frozen section to examine the expression and function of tight junctions in the epithelium of human palatine tonsils from patients with tonsillar hypertrophy or recurrent tonsillitis
|Go M,Kojima T,Takano K,Murata M,Ichimiya S,Tsubota H,Himi T,Sawada N||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:1627)||2004|